A method for rapid propagation of plantain semi-liquid in vitro tissue culture

Abstract

The invention discloses a semiliquid in-vitro tissue culture and rapid propagation method for MuSa basjoo. According to the method, a semiliquid induction medium comprises an MS culture solution, 6-BA with the concentration of 4 mg / L, NAA with the concentration of 0.15 mg / L, sucrose with the concentration of 30 g / L, activated carbon with the concentration of 0.5 g / L and agar with the concentration of 3 g / L, explants are cultured in a constant-temperature shaking incubator with the shaking frequency of 30 times per minute under the conditions that pH is 5.6-5.8, the temperature is 28-30 DEG C, the illumination intensity is 1,600-2,000 lx and the illumination time of 8 h / d; a multiplication subculture medium mainly comprises the MS culture solution, 6-BA with the concentration of 3 mg / L, NAA with the concentration of 0.15 mg / L and the activated carbon with the concentration of 0.5 g / L; a rooting medium mainly comprises the MS culture solution, NAA with the concentration of 0.5 mg / L, IBA with the concentration of 0.1 mg / L and the activated carbon with the concentration of 0.5 g / L. The browning ratio of the MuSa basjoo is reduced, the induced differentiation ratio and the multiplication coefficient are increased remarkably, and the culture period is shortened.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of Saigon banana, and in particular relates to a semi-liquid rapid in vitro propagation method of Saigon banana. Background technique [0002] Banana (MuSa basjoo), also known as MuSa abb, is one of the edible bananas in the Musa family. Saigon banana has beautiful fruit shape, thin skin and bright yellow color, tender flesh, suitable sweetness, good commerciality, and strong adaptability to barrenness, drought and cold. It is very popular in the market for its resistance to banana leaf spot, bunchy top, mosaic core rot and annual fruiting. It has been widely planted in Guangxi, Guangdong, Yunnan and other places, and has achieved good economic and social benefits, which has increased the enthusiasm of banana farmers to plant Saigon bananas. Traditional seedling propagation is difficult to meet the demand for banana seedlings in the market. With the market With the development of the econ...

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Problems solved by technology

[0004] In order to solve the technical problems of high explant browning rate, slow bud proliferation and long growth cycle in the tissue culture and rapid propagation of plantain in existing tissue culture technology, the invention provides a semi-liquid isolated tissue culture and rapid propagation method of plantain, To meet the needs of large-scale production of high-quality plantain seedlings