The preparation method of the protoplast of Gracilaria gracilaria in Chile
A technology for protoplasts and gracilis, which is applied in the field of preparation of gracilaria protoplasts in Chile, and can solve problems such as poor enzymatic hydrolysis effect and the like
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0016] Preparation of tissue enzyme solution: homogenate it with seawater 0°C with 2 times the volume of sea cucumber intestine; extract at 4°C for 12 hours, then adjust the pH value of the extract to 7.6, refrigerate and centrifuge for 5 minutes, and take the supernatant. Sea cucumber enzyme solution; tissue enzyme solution is obtained by mixing sea cucumber enzyme solution, cellulase and seawater. The specific composition of the tissue enzyme solution is 2.4ml cellulase, 0.5ml sea cucumber enzyme solution and 17.1ml sterilized seawater.
[0017] Preparation of protoplasts: Take 2g of Chilean river fence material, blot dry with absorbent paper, chop the algae with a scalpel (the more broken the better), add the prepared tissue enzyme solution, and put it on a constant temperature shaker at 25°C Dark enzymolysis. Microscopically check once every 30 minutes, observe and take pictures. After 5 hours, filter with a 200-mesh sieve to remove undigested cells, cell clusters and de...
Embodiment 2
[0019] Detection of protoplasts:
[0020] Determination of Cell Density: Determination of Cell Density Using a Hemocytometer
[0021] The specific results are as follows:
[0022]
[0023]
Embodiment 3
[0033] The protoplasts prepared in Example 1 were collected and cultured under low light in hypertonic seawater with an illumination intensity of 100-200lx. After the protoplast regenerated the wall, the osmotic pressure of seawater was gradually reduced to normal seawater, and the illumination intensity was gradually increased to 3000lx for cultivation.
[0034] Such as figure 2 As shown, after 42 hours of culture, the protoplasts have not yet formed a complete cell wall, and after 4 days of culture, most of the protoplasts have regenerated their cell walls. After 7 days of culture, the cells have been divided into multiple cells, and the cells are arranged more regularly. After 12 days of culture, the number of cells has been increasing and they are arranged in different shapes.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com