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Agar culture medium and manufacturing method thereof

A kind of technology of agar medium and culture medium, applied in the direction of plant cells, etc., can solve the problems of easy paste of agar and difficult to avoid bacterial contamination, and achieve the effect of avoiding temperature rise and pressure, and avoiding secondary pollution

Inactive Publication Date: 2014-03-26
QINGDAO WINCHANCE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commercially available agar medium is mainly used in the cultivation, isolation or identification of microorganisms, and is mainly prepared by heating, boiling and dispensing. If the agar is not stirred evenly during the long-term heating and boiling process, it is very easy to paste the bottom, and it will be separated after boiling. It is also difficult to avoid bacterial contamination in the way of packaging

Method used

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  • Agar culture medium and manufacturing method thereof

Examples

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Effect test

Embodiment 1

[0014] An agar medium, its composition and mass number are: per liter of PES medium: 25g of sucrose, 6g of agar, 8mg of ascorbic acid, 11mg of vitamin E, 2mg of IAA, 1mg of ZT.

[0015] Dissolve sucrose, ascorbic acid, vitamin E, IAA and ZT in 1L of PES medium, adjust its pH to 7.2-7.8, and adjust its salinity to 28-30, then divide it into 20 three-necked conical flasks, and put 6g of agar Add to each Erlenmeyer flask on average, add 0.3g agar to each Erlenmeyer flask, seal with parafilm and sterilize at high temperature and high pressure in a pressure cooker for 20min, take out the Erlenmeyer flask and place it on a flat surface to cool until the agar solidifies.

Embodiment 2

[0017] An agar medium, its composition and mass number are: per liter of PES medium: 20g of sucrose, 5g of agar, 5mg of ascorbic acid, 10mg of vitamin E, 1mg of IAA, 1mg of ZT.

[0018] Dissolve sucrose, ascorbic acid, vitamin E, IAA and ZT in 1L of PES medium, adjust its pH to 7.2-7.8, and adjust its salinity to 28-30, then divide it into 20 three-necked conical flasks, and put 5g of agar Add to each Erlenmeyer flask on average, add 0.25g agar to each Erlenmeyer flask, seal with parafilm and sterilize at high temperature and high pressure in a pressure cooker for 20min, take out the Erlenmeyer flask and place it on a flat surface to cool until the agar solidifies.

Embodiment 3

[0020] An agar medium, its composition and mass number are: per liter of PES medium: 40g of sucrose, 10g of agar, 8mg of ascorbic acid, 15mg of vitamin E, 4mg of IAA, 4mg of ZT.

[0021] Dissolve sucrose, ascorbic acid, vitamin E, IAA and ZT in 1L of PES medium, adjust its pH to 7.2-7.8, and adjust its salinity to 28-30, then distribute it in 20 three-necked conical flasks, and put 10g of agar Add to each Erlenmeyer flask on average, add 0.5g agar to each Erlenmeyer flask, seal with parafilm and sterilize at high temperature and high pressure in a pressure cooker for 20min, take out the Erlenmeyer flask and place it on a flat surface to cool until the agar solidifies.

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Abstract

The invention discloses an agar culture medium and a manufacturing method thereof. The agar culture medium is mainly used for inducing callus tissues in a red algae tissue culture process, wherein 20-40g of sucrose, 5-10g of agar, 5-10mg of ascorbic acid, 10-15mg of vitamin E, 1-4mg of IAA and 1-4mg of ZT are adding into every liter of PES culture medium; and the red algae is any of Gelidium amansii, Gelidiella, Gracilaria verrucosa, Gracilaria lemaneiformis, Chondrus crispus and Grateloupia filicina. A manner of agar subpackaging before high-pressure sterilization is adopted, thus avoiding scorch of the agar at the bottom due to nonuniform heating and avoiding secondary pollution during subpackaging.

Description

technical field [0001] The invention relates to an agar medium and a preparation method thereof, in particular to an agar medium used for inducing callus formation during tissue culture of red algae. Background technique [0002] Agar, also known as "agar", also known as agar, jelly powder, agar-agar extract, agar powder, is a polysaccharide extracted from red algae plants such as agaricus and Gracilaria, and its main component is polygalactose sulfate . Because of its special gel properties, agar has been widely used in food, medicine, chemical industry, textile, national defense, scientific research and other fields. Agar has long been listed as a generally safe product by the U.S. Food and Drug Administration Regulations, and has been approved as a food additive and listed in the Pharmacopoeia of Food Chemicals as a special topic. In 1973, the Food and Agriculture Organization of the United Nations and the World Health Organization evaluated that agar per person per kil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 苏建丽
Owner QINGDAO WINCHANCE TECH
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