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A kind of induction method of Gracilaria callus

A technique for callus and Gracilaria, applied in the field of Gracilaria callus induction, can solve the problems of low callus induction rate, backward plant tissue culture technology, difficulty in inducing callus, etc. The effect of quality resources

Active Publication Date: 2016-08-17
安徽省满林农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the disinfection technology, induction medium composition and culture conditions of Gracilaria are still in the exploratory stage, so the plant tissue culture technology of Gracilaria is still relatively backward, it is difficult to induce callus, or the induction rate of callus is extremely low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Remove the roots of Gracilaria and wash them. Soak the fronds of Gracilaria in detergent with a volume fraction of 0.2% for 5 minutes, clean them with sterile filtered seawater, and soak them in a mercuric chloride solution with a volume fraction of 1% for 3 minutes. After cleaning with sterile filtered seawater, put it into sterile filtered seawater and irradiate it under ultraviolet light for 10-min; cut the fronds of Gracilaria into 0.2-0.5mm sections, and inoculate 100 pieces in total for callus induction culture culture medium in the dark at 15-17°C for 7 days, then transfer the culture medium inoculated with Gracilaria to 20-22°C, and continue the shaker culture at 2000-3000Lx. The culture for inducing callus formation in Gracilaria was as follows: 2 mg of 2,4-D, 0.5 mg of IAA, 0.5 mg of KT, and 30 g of sucrose were added to 1 L of PESI medium; the rotation speed of shaker culture was 80 r / min. After one month of culture, 60 explants formed callus, and the callus ...

Embodiment 2

[0016] Remove the roots of Gracilaria and wash them. Soak the fronds of Gracilaria in detergent with a volume fraction of 0.2% for 5 minutes, clean them with sterile filtered seawater, and soak them in a mercuric chloride solution with a volume fraction of 1% for 3 minutes. After cleaning with sterile filtered seawater, put it into sterile filtered seawater and irradiate it under ultraviolet light for 10-min; cut the fronds of Gracilaria into 0.2-0.5mm sections, and inoculate 100 pieces in total for callus induction culture culture medium in the dark at 15-17°C for 7 days, then transfer the culture medium inoculated with Gracilaria to 20-22°C, and continue the shaker culture at 2000-3000Lx. The culture for inducing callus formation in Gracilaria was as follows: 2 mg of 2,4-D, 0.5 mg of IAA, 0.5 mg of KT, and 30 g of sucrose were added to 1 L of PESI medium; the rotation speed of shaker culture was 100 r / min. After one month of culture, 65 explants formed callus, and the callus...

Embodiment 3

[0018] Remove the roots of Gracilaria and wash them. Soak the fronds of Gracilaria in detergent with a volume fraction of 0.2% for 5 minutes, clean them with sterile filtered seawater, and soak them in a mercuric chloride solution with a volume fraction of 1% for 3 minutes. After cleaning with sterile filtered seawater, put it into sterile filtered seawater and irradiate it under ultraviolet light for 10-min; cut the fronds of Gracilaria into 0.2-0.5mm sections, and inoculate 100 pieces in total for callus induction culture culture medium in the dark at 15-17°C for 7 days, then transfer the culture medium inoculated with Gracilaria to 20-22°C, and continue the shaker culture at 2000-3000Lx. The culture for inducing callus formation in Gracilaria was as follows: 4 mg of 2,4-D, 1 mg of IAA, 1 mg of KT, and 30 g of sucrose were added to 1 L of PESI medium; the rotation speed of the shaker culture was 80 r / min. After one month of culture, 40 explants formed callus, and the callus ...

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Abstract

The invention discloses an inducing method of gracilaria verrucosa callus. The method is characterized by comprising the steps of inoculating gracilaria verrucosa thallus which is adopted as an explant and sterilized by a detergent and mercury bichloride in an inducing culture medium, and adding 2mg of 2,4-D, 1mg of IAA, 1mg of KT and 30g of cane sugar in a 1L PESI culture medium; inducing the explant for 7d in darkness, and continuously culturing the explant under the illumination condition to form a great amount of callus. By adopting the method, the callus can be successfully induced by adopting the gracilaria verrucosa as the explant, the induction rate of the callus can reach 82 percent, the callus can further develop to form bud or thallus of the gracilaria verrucosa, and not only can a great amount of gracilaria verrucosa thallus be formed, but also rich seed resource can be provided for the gracilaria verrucosa.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing callus of Gracilaria. Background technique [0002] Gracilaria ( Gracilaria verrucosa ) is a common red algae in our country. The algae contains a lot of agar and fatty acids. It is not only widely used in the agar production industry, but also a delicacy on people's tables. At present, Gracilaria is mainly based on wild resources, and the artificial cultivation technology is immature. The main factors limiting artificial breeding are the unstable source of seed algae and the lack of germplasm resources. At present, Gracilaria mainly adopts the method of spore propagation for artificial breeding. The breeding cycle is relatively long, and the spore germination rate is not high. Compared with the seed algae used for breeding, the yield of new algae obtained is not ideal. [0003] Plant tissue culture technology can reproduce rapidly, year-round...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 苏建丽
Owner 安徽省满林农业科技发展有限公司
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