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Ultrafine crushing method of cell walls of large alga Gracilaria verrucosa

A technology for fine crushing and cell wall, which is applied in the field of ultra-fine crushing of cell walls of large seaweed Astragalus, and achieves the effects of low processing cost, less labor required, and less production area occupied.

Active Publication Date: 2015-01-21
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the deepening of research, some wall-breaking technologies have also obtained better extraction results, but most of them are limited to the preparation of a small number of samples in the laboratory. Therefore, it is of great significance to find a fast and industrially-operable wall-breaking technology.

Method used

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  • Ultrafine crushing method of cell walls of large alga Gracilaria verrucosa
  • Ultrafine crushing method of cell walls of large alga Gracilaria verrucosa
  • Ultrafine crushing method of cell walls of large alga Gracilaria verrucosa

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Effect test

Embodiment 1

[0019] (1) Fresh dark reddish-brown thick asparagus-981 (981 asparagus) from Nan'ao Island, Shantou, Guangdong, or asparagus-981 stored at low temperature, washed with water and drained.

[0020] (2) Mix the above-mentioned asparagus with PBS buffer according to the ratio of weight to volume (W (g) / V (ml)), asparagus: PBS buffer = 1:2, mix and put into cylindrical ice Store in the freezer at -20°C for 5 hours.

[0021] (3) Adjust the ultra-fine crushing device so that the dihedral angle formed by the ice-shaving knife and the ice-shaving table is 50°-60°, and the vertical height between the blade of the ice-shaving knife and the ice-shaving table is less than 0.5mm.

[0022] (4) Place the above-mentioned frozen seaweed ice cubes in the above-mentioned adjusted ultra-fine crushing device for crushing.

[0023] (5) Collect the broken seaweed ice in the above step (4), melt the broken seaweed ice at 4°C and then freeze it again at -20°C, then repeat the above breaking, repeat 5 ...

Embodiment 2

[0026] (1) Fresh dark reddish-brown thick asparagus-981 from Nan'ao Island, Shantou, Guangdong Province or asparagus-981 stored at low temperature, washed with water and drained.

[0027] (2) Mix the above-mentioned asparagus with PBS buffer according to the ratio of weight to volume (W (g) / V (ml)), asparagus: PBS buffer = 1:2, mix and put into cylindrical ice Place in the tank at -80°C for 3 hours.

[0028] (3) Adjust the ultra-fine crushing device so that the dihedral angle formed by the ice-shaving knife and the ice-shaving table is 50°-60°, and the vertical height between the blade of the ice-shaving knife and the ice-shaving table is less than 0.5mm.

[0029] (4) Place the above-mentioned frozen seaweed ice cubes in the above-mentioned adjusted ultra-fine crushing device for crushing.

[0030] (5) Collect the broken seaweed ice in the above step (4). The crushed seaweed ice melts at 25°C and freezes again at -80°C, then repeats the above breaking. Repeat 3 times to compl...

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Abstract

The invention relates to a cell crushing technology, and concretely relates to an ultrafine crushing method of cell walls of large alga Gracilaria verrucosa. The method comprises the following steps: mixing Gracilaria verrucosa with a PBS buffer solution according to a weight / volume ratio of 2-4:1 (W / V), and carrying out freezing-crushing treatment 3-5 times to realize complete wall breaking of the alga; and diluting the alga paste obtained after the wall breaking to 10 times, and extracting for 48h to realize the phycoerythrin purity of 0.35 and the leaching rate of 43.91% (mg / g), wherein the diameters of alga particles in the alga paste generally reach 0.1-0.5mm. The method has the advantages of simple device, simple operation, high wall breaking rate, benefiting of the dissolving-out of biologically active substances, and the like.

Description

technical field [0001] The invention relates to cell crushing technology, in particular to an ultrafine crushing method for cell walls of large seaweed asparagus. Background technique [0002] The large seaweed Asparagus is rich in phycoerythrin and bromoperoxidase. As the main raw material for extracting phycoerythrin and bromoperoxidase, it is also widely planted in my country. Since the improved new variety "Asparagus-981" has been cultivated on a large scale in Nan'ao, Shantou, Guangdong, asparagus has become the third largest algae cultivation industry after kelp and seaweed. The average yield per mu has reached 3.5 tons, and the national planting area has reached 20. This provides the possibility for large-scale separation and purification of phycoerythrin and bromoperoxidase. [0003] Asparagus algae are upright, linear, cylindrical, clustered, 30-50cm high, up to 1m or more in length. Phycoerythrin and bromoperoxidase in asparagus are intracellular substances. To f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B02C18/00
CPCB02C18/00
Inventor 刘冰谷洋洋杜虹秦松麦伟群陈伟洲
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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