A method for tissue culture and rapid propagation of Polygonatum longiflorum

A tissue culture, Polygonatum chinensis technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., to achieve the effects of reducing pretreatment time, low pollution rate, and beneficial to industrial production

Active Publication Date: 2019-06-28
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture and rapid propagation of Polygonatum long stems has not been reported yet

Method used

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  • A method for tissue culture and rapid propagation of Polygonatum longiflorum
  • A method for tissue culture and rapid propagation of Polygonatum longiflorum
  • A method for tissue culture and rapid propagation of Polygonatum longiflorum

Examples

Experimental program
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Embodiment 1

[0029] A method for tissue culture and rapid propagation of Rhizoma Polygonatum, comprising the following steps in turn:

[0030] (1) Disinfection of Polygonatum long-stemmed flower buds

[0031] In May of that year, take the flower buds of Polygonatum long-stemmed that have not yet bloomed, put them in the refrigerator at 4°C for 12-24 hours, add an appropriate amount of detergent solution to soak for 2 minutes, wash them with running water for 0.5-1.0 hours, and put them in the ultra-clean bench Turn the stem section into 70% alcohol solution and soak for 1min, rinse it with sterile water for 3 times, and dip it in 5 drops of Tween-80, the mass fraction is 0.1% HgCl 2 The solution is soaked and disinfected for 9-10 minutes, and then fully soaked with sterile water for 3 times to obtain the sterilized explant material, and the success rate of disinfection can reach more than 90%.

[0032] (2) Induction of clustered buds

[0033] Put the explant material obtained in the prev...

Embodiment 2

[0041] The difference between this example and Example 1 lies in step (1): Disinfection of Polygonatum long-stemmed flower buds: In May of that year, take the flower buds of Polygonatum long-stemmed that have not yet bloomed, put them in the refrigerator at 4°C for 0-48h, take them out and add them Soak in an appropriate amount of detergent solution for 2 minutes, rinse with running water for 0.5-1.0 hours, then transfer the stem segment to 70% alcohol solution and soak for 1 minute in an ultra-clean bench, rinse with sterile water for 3 times, immerse in 5 drops of Drip Tween-80, HgCl with a mass fraction of 0.1% 2 The solution is soaked and disinfected for 9-10 minutes, and then fully soaked with sterile water for 3 times to obtain the sterilized explant material. It can be seen from the statistical results 30 days after inoculation that the pollution rate obtained by using flower buds as explants for disinfection is far lower than that of using rhizomes as explants, and the...

Embodiment 3

[0045] The difference between this embodiment and Example 1 is that step (2): the induction of clustered buds: put the explant material obtained in the previous step on sterile filter paper, cut off the head and tail, remove the stamens and pistils, and cut the petals into 2cm 2 Size, in the MS basic medium with TDZ 0-2.0mg / L, NAA 0-1.0mg / L, AC 0-2.0g / L, the amount of sucrose in the medium is 30g / L, agar The amount is 8.0g / L, the pH is 5.8-6.0, and the medium has been sterilized at 121°C for 20min, the same below. The petal-connected culture medium was first cultured in the dark for 0-7 days, and the culture temperature was 28±2°C; then it was transferred to light culture, and the culture conditions were as follows: the culture temperature was (28±2)°C, and the light time was 14h light / 10h dark, light intensity 30-40μmol / (m 2 s); after 30d, statistics found that; the different treatments of TDZ concentration showed that low concentration TDZ (0.1mg / L) had lower adventitious...

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Abstract

The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rapid propagation of Polygonatum long-stalked. A method for tissue culture and rapid propagation of Polygonatum long-stemmed Polygonatum, the method comprising the following steps: (1) disinfection and inoculation of flower buds of Polygonatum long-stemmed; (2) induction of clustered buds; (3) proliferation of adventitious buds; (4) Rooting culture of regenerated plants; (5) Hardening and transplanting. By adopting the method of the present invention, compared with the reported literature, the pretreatment time can be shortened, the explant contamination rate is low, and the adventitious bud generation time can be shortened, thereby obtaining long-stalked Polygonatum regenerated plants faster, which is beneficial to industrial production. By adopting the method of the present invention, the induction rate of adventitious buds of long-stemmed Polygonatum polygonatum clustered buds is as high as 90.0%, the number of adventitious buds is 8.4, the multiplying multiple of adventitious buds can reach 7.7, and the survival rate of transplanted plants can reach more than 95%.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rapid propagation of Polygonatum long-stalked. Background technique [0002] Polygonatum filipes Merr. is a perennial herb of Liliaceae Polygonatum, distributed in Jiangsu, Anhui, Zhejiang, Jiangxi, Hunan, Fujian, Guangdong (northern), and grows in the shade of forests, shrubs or grass slopes. In wet and fertile soil, it is grown in large quantities because of its rhizomes and is used as medicine in the local area, and it is also edible. At present, seeds and rhizomes are used for the propagation of Polygonatum long-stemmed Rhizoma in production, mainly based on rhizomes, which have the disadvantages of low reproduction coefficient and long propagation cycle, so establishing a tissue culture and rapid propagation system for Polygonatum long-stemmed Another effective way for its industrialized production. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙骏威赵进周荣鑫
Owner CHINA JILIANG UNIV
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