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A nitrogen-sulfur-doped graphene quantum dot and its preparation method and its application in the preparation of lysine fluorescence detection reagent

A graphene quantum dot and fluorescence detection technology, applied in the field of fluorescence detection, can solve the problems of cumbersome and inconvenient detection of lysine, and achieve the effects of excellent biocompatibility, good water dispersibility, and easy-to-obtain products.

Active Publication Date: 2019-05-03
WENZHOU MEDICAL UNIV
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  • Description
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  • Application Information

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Problems solved by technology

[0003] In order to solve the cumbersome and inconvenient problem of the existing lysine detection, the present invention provides a nitrogen-sulfur-doped graphene quantum dot and its preparation method and its application in the fluorescence detection of lysine

Method used

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  • A nitrogen-sulfur-doped graphene quantum dot and its preparation method and its application in the preparation of lysine fluorescence detection reagent
  • A nitrogen-sulfur-doped graphene quantum dot and its preparation method and its application in the preparation of lysine fluorescence detection reagent
  • A nitrogen-sulfur-doped graphene quantum dot and its preparation method and its application in the preparation of lysine fluorescence detection reagent

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preparation example Construction

[0017] The preparation method of nitrogen-sulfur-doped graphene quantum dots is as follows: add citric acid and thiourea to a three-neck flask, add an appropriate amount of deionized water 5-35 mL, and stir to form a homogeneous solution. The mixed solution was transferred to a polytetrafluoroethylene-lined hydrothermal kettle. The mass ratio of citric acid and thiourea is 1:0.2~1:3, the heating time is 2~20 h, and the heating temperature is 130~230°C. The obtained product was dialyzed for three days with a dialysis bag with a molecular weight of 1000 to obtain the nitrogen-containing and sulfur-doped graphene quantum dots.

[0018] Preparation of lysine fluorescence detection reagent: the graphene quantum dots doped with nitrogen and sulfur are dissolved in water or alcohol aqueous solution, the aqueous solution is more practical and easy to use, and formulated to a certain concentration. The concentration can be determined according to the test conditions and the degree of ...

Embodiment 1

[0020] In fluorescence experiments, graphene quantum dots doped with nitrogen and sulfur were prepared as a 1.0 mg / mL aqueous stock solution, and amino acids and sugar molecules were selected from lysine, alanine, proline, glycine, methionine, and arginine , leucine, glucose, glucosamine, galactose, sucrose and other substances, all experimental solutions were newly configured and tested immediately. Excited at 340 nm, emitted at 440 nm. Take 2.5 mL of stock solution, add 2.5*10 -2 M amino acid or sugar solution. Test its fluorescence spectrum. see attached results figure 1 .

Embodiment 2

[0022] In the fluorescence experiment, the nitrogen-sulfur-doped graphene quantum dots were formulated into a 0.04 mg / mL aqueous solution. Lysine dubbed 2.5*10 -2 Standard stock solution of M. Alanine, proline, glycine, methionine, arginine, leucine, glucose, glucosamine, galactose, sucrose and other substances are selected as interfering amino acids and sugar molecules. All experimental solutions were freshly prepared and tested immediately. In the interfering substance experiment, 4 times of interfering ions were added to the aqueous solution of 0.04 mg / mL nitrogen-sulfur-doped graphene quantum dots to measure its fluorescence, and then lysine was added to measure its fluorescence change. Excitation at 340 nm, detection of fluorescence changes at 440 nm. figure 2 In each group, the lower ones of the rods are the corresponding interference ions, and the higher ones are the corresponding ones after adding lysine.

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Abstract

The invention relates to a nitrogen / sulfur-doped graphene quantum dots and a preparation method thereof, and application of quantum dots in preparing lysine fluorescence detection reagents. By adopting the product of the thiocarbamide / citric acid hydrothermal reaction, the nitrogen / sulfur-doped graphene nano quantum dots have the multiple electron characteristic, have the advantages of favorable water dispersibility, excellent biocompatibility, rich oxygen-containing functional groups (-COOH, -OH and -O-), high luminescence and high quantum efficiency. The quantum dots have high selectivity and sensitivity to lysine. The synthesis method is simple, and has the advantages of mild conditions and accessible product. When being used for testing lysine, the quantum dots are free of influence of other conventional coexisting amino acids and saccharide biomolecules (such as alanine, proline, glycinate, methionine, arginine, leucine, glucose, glucosamine, galactose, sucrose and the like), and have high selectivity.

Description

technical field [0001] The invention relates to the technical field of recognition and binding and fluorescence detection for optical detection of lysine, in particular to a nitrogen-sulfur-doped graphene quantum dot and its preparation method and its application in the preparation of lysine fluorescence detection reagents. Background technique [0002] Lysine is an essential amino acid that is widely used in medicine, food processing and agriculture. It has numerous physiological functions, such as participating in the synthesis of polyamines and the Krebs–Henseleit cycle. An imbalance in lysine diet, like some inborn metabolic diseases, can cause cystinuria or hyperlysinemia. Traditional detection methods such as: high performance liquid chromatography (HPLC), capillary electrophoresis (CE), flow injection chemiluminescence and chromatography. But these methods require complex preprocessing, high cost and professional personnel. Great progress has been made in the detect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65G01N21/64
CPCC09K11/65G01N21/6428
Inventor 程如梅葛聪聪陈路呀张立树王狄狮
Owner WENZHOU MEDICAL UNIV
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