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Preparation method of starch nanoparticle with controllable morphology and particle size

A technology of starch nano and granules, applied in the field of nano, which can solve the problems of uncontrollable particle size and shape, wide distribution of nano particle size, etc., and achieve the effects of low equipment requirements, short reaction time and high efficiency

Active Publication Date: 2017-06-23
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of wide particle size distribution and uncontrollable particle size and shape of nanoparticles prepared by existing methods, the present invention aims to provide a method for preparing starch nanoparticles with controllable shape and particle size

Method used

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  • Preparation method of starch nanoparticle with controllable morphology and particle size
  • Preparation method of starch nanoparticle with controllable morphology and particle size
  • Preparation method of starch nanoparticle with controllable morphology and particle size

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Prepare starch nanoparticles with different shapes and particle sizes according to the following steps:

[0036] (1) Preparation of buffer solution: Accurately weigh 3.27g of disodium hydrogen phosphate and 2.24g of citric acid and dissolve them in 200mL of distilled water, stir to fully dissolve, and set aside;

[0037] (2) Pullulanase pretreatment: Accurately measure 1 mL of pullulanase with a concentration of 1350 NPUN / mL and drop it into 10 mL of distilled water, stir to make it fully mixed, and set aside;

[0038] (3) Preparation of starch milk: Weigh 15 g of waxy cornstarch and add it to 100 mL of buffer solution to obtain a starch emulsion with a mass volume ratio of 15%;

[0039] (4) Gelatinization: put the prepared starch milk in a water bath at 100°C for 20 minutes to completely gelatinize the starch, and then cool down to 58°C;

[0040] (5) Enzymolysis: add 1.5 mL of treated pullulanase to the gelatinized colloidal starch solution, and enzymatically hydrolyz...

Embodiment 2

[0073] (1) Preparation of buffer solution: Accurately weigh 1.635g of disodium hydrogen phosphate and 1.12g of citric acid and dissolve in 100mL of distilled water, stir to fully dissolve, and set aside;

[0074] (2) Pullulanase pretreatment: Accurately measure 1 mL of pullulanase with a concentration of 1350 NPUN / mL and drop it into 9 mL of distilled water, stir to make it fully mixed, and set aside;

[0075] (3) Preparation of starch milk: Weigh 10 g of waxy cornstarch and add it to 100 mL of buffer solution to prepare a starch emulsion with a mass volume ratio of 15%;

[0076] (4) Gelatinization: Put the prepared starch milk in a water bath at 100°C for 40 minutes to completely gelatinize the starch, then cool down to 55°C;

[0077] (5) Enzymolysis: add 1 mL of the treated pullulanase to the gelatinized colloidal starch solution, and enzymatically hydrolyze at 55°C for 6 hours;

[0078] (6) Low-speed centrifugation: centrifuge the solution obtained while hot at a speed of ...

Embodiment 3

[0084] (1) Preparation of buffer solution: Accurately weigh 6.54g of disodium hydrogen phosphate and 4.48g of citric acid and dissolve in 400mL of distilled water, stir to fully dissolve, and set aside;

[0085] (2) Pullulanase pretreatment: Accurately measure 1 mL of pullulanase with a concentration of 1350 NPUN / mL and drop it into 8 mL of distilled water, stir to make it fully mixed, and set aside;

[0086] (3) Preparation of starch milk: Weigh 20 g of waxy cornstarch and add it to 100 mL of buffer solution to obtain a starch emulsion with a mass volume ratio of 20%;

[0087] (4) Gelatinization: put the prepared starch milk in a water bath at 100°C for 50 minutes to completely gelatinize the starch, and then cool down to 60°C;

[0088] (5) Enzymolysis: add 2 mL of the treated pullulanase to the gelatinized colloidal starch solution, and enzymatically hydrolyze at 60°C for 6 hours;

[0089] (6) Low-speed centrifugation: centrifuge the solution obtained while hot at a speed o...

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Abstract

The invention discloses a preparation method of a starch nanoparticle with controllable morphology and particle size by adopting natural polysaccharide as a template. The method comprises the steps of utilizing a bio-enzyme method for preparing a starch short linear chain, in-situ adding the natural polysaccharide template during a process for preparing the starch nanoparticle through short linear chain self-assembly; screening polysaccharide templates with different molecular structures, controlling the morphology and the particle size of the starch nanoparticle, and preparing to obtain the nanoparticle with controllable morphology (a hollow body, a spindle body and a sphere body) and particle size (20 to 120nm) suitable for different demands. The preparation method provided by the invention is low in equipment requirement, simple in process, simple and convenient to operate, mild in reaction, short in reaction time, high in efficiency, and suitable for mass production. The preparation method provided by the invention is low in preparation cost, less in energy consumption, and free of producing hazardous wastes, and meets the modern production requirements on green production, environmental protection and energy conservation.

Description

technical field [0001] The invention belongs to the field of nanotechnology, in particular to a method for preparing starch nanoparticles with controllable morphology and particle size. Background technique [0002] Starch nanoparticles are starch granules with a particle size of less than 300nm. At present, the preparation of starch nanoparticles mainly adopts chemical and physical methods, and acid hydrolysis is the most common method, mainly using H 2 SO 4 Or HCl hydrolyzes the amorphous region of starch granules to obtain starch nanocrystals. Starch nanoparticles can also be prepared by methods such as anti-solvent, high-pressure homogenization, and cross-linked microemulsion, but the above methods have low yield, high energy consumption, and high cost. Short amylose starch is obtained by debranching with biological enzymes, and starch nanoparticles are prepared by self-assembly, which has high yield, low energy consumption, and is green and safe. However, the size di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L3/12C08L5/08C08L5/00C08L5/04C08L93/04C08B30/20C12P19/16C12P19/04B82Y40/00
CPCB82Y40/00C08B30/20C08L3/12C12P19/04C12P19/16C08L5/08C08L5/00C08L5/04C08L93/04
Inventor 孙庆杰熊柳李晓静邱超卢浩
Owner QINGDAO AGRI UNIV
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