Purification method for decoloring and deproteinizing Scholar bean polysaccharide

Abstract

The invention discloses a purification method for decoloring and deproteinizing Scholar bean polysaccharide. The method comprises the following steps: extracting Scholar beans to obtain Scholar bean crude polysaccharide; performing pretreatment by D113 weakly acidic cation exchange resin, repeatedly rinsing with deionized water until water is clear and not brown, selecting a glass chromatography column, packing the column by a wet method, washing with alkali and deionized water until the pH value is 7, washing with acid and deionized water until the pH value is 7, and soaking with deionized water for future use; adopting D113 weakly acidic cation exchange resin, adding Scholar bean crude polysaccharide with the mass concentration of 4.0 to 4.5 mg / mL, the pH value of 5.3 to 5.8 and the BV volume of 2.7 to 3.2, performing operation at a flow rate of 1.4 to 1.9 mL / min, deproteinizing, decoloring and retaining polysaccharide. The method is simple in operating step, little in influence on the polysaccharide structure, low in cost, environmentally-friendly, rapid in adsorption and high in capacity, and the resin can be reused.

Description

technical field [0001] The invention relates to a purification method for the decolorization and deproteinization of the champion bean polysaccharide. Background technique [0002] At present, the decolorization and deproteinization methods of polysaccharides mainly include: enzymatic method, organic reagent method (Sevage method, trichloroacetic acid method, etc.), adsorption method (activated carbon method, macroporous resin method, etc.), hydrogen peroxide oxidation method, etc. Among them, the organic reagent method has problems such as many repetitions and large amount of reagents, the enzymatic method has problems such as high cost, and high inactivation temperature easily destroys the structure of polysaccharides and destroys the activity, hydrogen peroxide easily causes polysaccharides to degrade and other problems, and activated carbon has problems such as decolorization Long time, large loss of polysaccharides and other problems. The macroporous resin method mainl...

AI Technical Summary

Problems solved by technology

Among them, the organic reagent method has problems such as many repetitions and a large amount of reagents, the enzymatic method has problems such as high cost, and the high inactivation temperature easily destroys the structure of polysaccharides and destroys the activity, hydrogen peroxide easily causes problems such as polysaccharide degradation, and activated carbon has decolorization Long time, large loss of polysaccharides, etc.

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