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Application of aloperine to preparation of radiotherapeutic sensitizer for lung cancer

A kind of technology of sophorine and sensitizer, applied in the field of sophorine, and can solve the problems such as no somarine, research discovery and the like

Inactive Publication Date: 2017-06-23
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research on the role of aloperine in lung cancer radiotherapy resistance.

Method used

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  • Application of aloperine to preparation of radiotherapeutic sensitizer for lung cancer
  • Application of aloperine to preparation of radiotherapeutic sensitizer for lung cancer
  • Application of aloperine to preparation of radiotherapeutic sensitizer for lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0025] Example 2 Aloperine causes G1 / S phase arrest in lung cancer radiotherapy resistant cell lines

[0026] Cell proliferation is affected by the cell cycle, and studies have found that cell cycle checkpoint molecules CDK4, CKD6 and CyclinD1 regulate the transition of cells from G1 phase to S phase, and CDC2 regulates the transition of cells from G2 phase to M phase.

[0027]The experimental method is as follows: A549 / IR cells were treated with 6Gy radiotherapy for 1 hour, 10mM aloperine for 24 hours, and 6Gy radiotherapy combined with aloperine for 24 hours. The influence of Western blot to detect the expression of cell cycle checkpoint molecules CDK4, CKD6, CyclinD1, CDC2.

[0028] The results showed that aloperine alone or in combination with radiotherapy could lead to G1 / S phase arrest of lung cancer radiotherapy-resistant cells A549 / IR. At the same time, it significantly inhibited the protein expression levels of G1 / S phase checkpoint molecules CDK4, CKD6, and CyclinD1...

Embodiment 5

[0036] Example 5 Inhibition of autophagy enhances the toxic effect of radiotherapy on radiotherapy-resistant cells of lung cancer

[0037] The experimental method is as follows: A549 / IR cells were treated with 6Gy radiotherapy for 1h, 10mM aloperine combined with 6Gy radiotherapy for 24h, 10mM chloroquine combined with 6Gy radiotherapy for 24h, 6Gy radiotherapy and aloperine / chloroquine combined treatment for 24h, or in Under the conditions of 6Gy radiotherapy for 1 hour, 10mM aloperine combined with 6Gy radiotherapy for 24 hours, 10mM BafA combined with 6Gy radiotherapy for 24 hours, 6Gy radiotherapy and aloperine / BafA combined treatment for 24 hours, the total protein was extracted for Western Blot analysis of Beclin1 and p62 . Take 1×10 3 Cells in good condition were seeded on a 96-well plate, and 20 μl of MTS reagent was added, incubated for 0, 24, 48, and 72 hours, and the absorbance value was measured at a wavelength of 490 nm to calculate the cell proliferation rate. ...

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Abstract

The invention discloses application of aloperine to the preparation of an autophagic level inhibitor and a radiotherapeutic sensitizer for a radiotherapeutically resistant lung-cancel cell. The aloperine is a natural compound of a plant origin; the radiotherapeutic sensibilization mechanism of the aloperine is mainly to change biomarkers Beclin1, LC3 and p62, preferably fluorescently visual LC3 (Ad-mRFP-GFP-LC3), which are related to the autophagic level of a cell. Through detecting those specific biomarkers, those specific biomarkers are used for evaluating the autophagic level inhibition and a radiotherapeutic sensitization effect of the aloperine on the radiotherapeutically resistant lung-cancel cell.

Description

technical field [0001] The invention relates to aloperine that inhibits cell proliferation, enhances the radiotherapy sensitivity of lung cancer, and regulates the autophagy level of cells. By detecting some specific biomarkers, it is used to evaluate the anti-radiotherapy-resistant autophagy level of lung cancer cells and the radiotherapy of aloperine. Sensitization effect. Background technique [0002] Lung cancer (lung cancer) is the main cause of death of cancer patients in the world, and about 1.6 million lung cancer patients die every year. Clinically, radiotherapy has become one of the main treatment methods for lung cancer patients. However, recent studies have found that tumor cells gradually develop resistance to the cytotoxic effect caused by radiotherapy, resulting in significantly worsened radiotherapy efficacy and survival prognosis for lung cancer. Therefore, elucidating the regulatory mechanism of lung cancer cell radiotherapy resistance will help to discove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/4748A61K45/06A61K31/4706A61K31/7048A61P35/00C12Q1/02G01N21/64G01N33/50
CPCA61K31/4706A61K31/4748A61K31/7048A61K41/0038A61K45/06C12Q1/02G01N21/6486G01N33/5011A61K2300/00
Inventor 龚志成徐志杰颜元良熊小明钱龙曾双双戴爽
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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