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Clinic detection marker of inflammatory bowel disease, and applications thereof

An inflammatory bowel disease and marker technology, applied in the application field of preparing inflammatory bowel disease products, can solve problems such as unreported, and achieve the effects of improving integrity, relieving symptoms, and reducing inflammatory cell infiltration

Active Publication Date: 2017-06-20
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report about the use of Erbin as a molecular marker for the diagnosis of inflammatory bowel disease and the use of autophagy inhibitors as a drug to inhibit inflammatory bowel disease

Method used

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  • Clinic detection marker of inflammatory bowel disease, and applications thereof
  • Clinic detection marker of inflammatory bowel disease, and applications thereof
  • Clinic detection marker of inflammatory bowel disease, and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Experiment of Erbin as a diagnostic marker for inflammatory bowel disease

[0025] 1. Construction of IBD animal model

[0026] Dextran sodium sulfate (DSS) chemically induced mouse IBD model was given to 20 8-week-old C57 mice and Erbin + / + and Erbin - / -Mice were fed with a concentration of 5%, DSS solution with a molecular weight of 36000-40000, and drank freely for 7 days to construct a mouse model simulating human ulcerative colitis; also fed 20 Erbin systemic deletion mice (Erbin - / - ) and 20 Erbin + / + The mice were given normal diet and drinking water, and the mice were sacrificed 7 days later, and the colorectum was taken.

[0027] 2. Il-10 - / - Spontaneous chronic enteritis mouse model

[0028] Il-10 - / - Mice fed with SPF for 6 months developed spontaneous enteritis, manifested as blood in the stool and rectal prolapse. In HE slices, we observed a large number of infiltration of inflammatory cells, and a large number of intestinal epithelial prol...

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Abstract

The invention relates to a clinic detection marker of an inflammatory bowel disease, and applications thereof, and concretely relates to a molecular marker-Erbin gene in the inflammatory bowel disease, and an application of an inhibitor chloroquine of a downstream autophagy pathway regulated by the marker. An experiment result shows that the intestinal mucosa damage of DSS induced Erbin<+ / +> and Erbin<- / -> mice injected with chloroquine are substantially reduced, the intestinal mucosa damage of the Erbin<- / -> mice is obviously severer than that of the Erbin<+ / +> mice, and the mice injected with chloroquine have higher intestinal mucosa integrity, substantially lower inflammatory cell infiltration and substantially alleviated inflammatory bowel disease symptoms than mice not injected with the chloroquine, so the autophagy inhibitor chloroquine can improve the integrity of the intestinal mucosa and has a very good IBD inhibition effect, thereby the chloroquine has certain prevention and treatment effects on the inflammatory bowel disease.

Description

technical field [0001] The invention belongs to the field of molecular diagnosis, and relates to a molecular marker for the diagnosis of inflammatory bowel disease and its application, in particular to the molecular marker-Erbin gene in inflammatory bowel disease, and the inhibition of the downstream autophagy pathway regulated by it Application of chloroquine in the preparation of inflammatory bowel disease products. Background technique [0002] Inflammatory bowel disease (IBD), which mainly includes Crohn's disease (CD for short) and ulcerative colitis (UC for short), is a chronic, nonspecific intestinal inflammatory disease. An important precancerous lesion of colorectal cancer. The main factors affecting the occurrence and development of IBD include intestinal immune system dysfunction, intestinal microbial infection, intestinal microecological disorder, and the genetic background of the host. However, the pathogenesis and key molecules of IBD are still far from eluci...

Claims

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Application Information

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IPC IPC(8): G01N33/68C12Q1/68A61K31/4706A61P35/00A61P1/00A61P29/00
CPCA61K31/4706C12Q1/6883C12Q2600/136C12Q2600/158G01N33/68
Inventor 李建明沈彤李师蔡令栋
Owner SUZHOU UNIV
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