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Flunixin meglumine monoclonal antibody hybridoma cell strain YY and application thereof

A technology of flunixin meglumine and hybridoma cell lines, applied in biochemical equipment and methods, material inspection products, instruments, etc., can solve problems such as low sensitivity, difficulty in obtaining accurate results, lengthy separation and purification process, etc., and achieve The effect of good specificity and good detection sensitivity

Active Publication Date: 2017-06-20
JIANGNAN UNIV
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Problems solved by technology

In order to effectively supervise and monitor the clinical use of flunixin meglumine in veterinary medicine, it is necessary to find a method with good specificity and high sensitivity. However, current detection methods such as thin-layer chromatography, gas chromatography, and liquid chromatography etc., the separation and purification process is lengthy, the sensitivity is low, and there are many interfering substances, it is difficult to obtain accurate results

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  • Flunixin meglumine monoclonal antibody hybridoma cell strain YY and application thereof

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Embodiment 1

[0018] Embodiment 1 Preparation of hybridoma cell line YY

[0019] (1) Preparation of complete antigen: Weigh 2.2 mg FM, 2.6 mg 1-ethylcarbodiimide hydrochloride, 1.6 mg N-hydroxysuccinimide, and dissolve with 400 μL anhydrous N,N-dimethylformaldehyde The amide was dissolved and stirred at room temperature for 6-8h (referred to as solution A). Take 10 mg of bovine serum albumin BSA (the molar ratio of FM to BSA is 30:1), and dissolve it with 4 mL of boric acid buffer solution (called solution B). At room temperature, add solution A to solution B dropwise, and react overnight at room temperature to obtain the conjugate FM-BSA mixture, and separate the complete antigen and unconjugated small molecule hapten by dialysis. Preparation of coated original FM-OVA: Weigh 1.6mg FM, 1.9mg 1-ethylcarbodiimide hydrochloride, 1.2mg N-hydroxysuccinimide, add 300μL anhydrous N,N-dimethyl Dissolve the formamide and stir at room temperature for 6-8 hours (referred to as liquid A). Weigh 5 mg...

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Abstract

The invention discloses a flunixin meglumine monoclonal antibody hybridoma cell strain YY and application thereof and belongs to the field of food safety immunoassay. The flunixin meglumine monoclonal antibody hybridoma cell strain YY disclosed by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC for short) with a preservation number of CGMCC No.13096. The monoclonal antibody secreted by the cell strain has better specificity and detection sensitivity (an IC50 value is 0.24ng / mL) to flunixin meglumine, provides raw materials for immunoassay of flunixin meglumine residues in food and has an actual application value.

Description

technical field [0001] The invention relates to a flunixin meglumine monoclonal antibody hybridoma cell line YY and an application thereof, belonging to the field of food safety immunoassay. Background technique [0002] Flunixin meglumine (flunixin meglumine, FM) is a new type of non-steroidal animal-specific antipyretic and analgesic drugs, of which, flunixin (flunixin, molecular formula C 14 h 11 f 3 N 2 o 2 ) is a special structure of a class of non-steroidal anti-inflammatory analgesic antipyretic drugs, meglumine (meglumine, molecular formula C 7 h 17 NO 5 ) mainly acts as a stabilizer, and the two appear in a fixed ratio (1:1, that is, one molecule of flunicin combined with one molecule of meglumine), which is FM. In veterinary medicine, it is often used to relieve horses' visceral colic, pain caused by muscle and bone disorders, and anti-inflammation; to control acute inflammation caused by various diseases and infections in cattle, such as laminitis, arthriti...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/44G01N33/577G01N33/94C12R1/91
CPCC07K16/44G01N33/577G01N33/9486
Inventor 胥传来林璐匡华徐丽广马伟刘丽强吴晓玲宋珊珊胡拥明
Owner JIANGNAN UNIV
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