A kind of tebuconazole molecularly imprinted membrane electrode, portable sensor and its use method and application

A technology of molecularly imprinted membrane and tebuconazole, applied in instruments, scientific instruments, analytical materials, etc., can solve the problems of contaminated sample test results, inaccurate test results, complicated operation, etc., achieve fast and accurate on-site detection, and improve sensitivity and accuracy, the effect of amplifying the response signal

Active Publication Date: 2019-06-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the limitations of these analysis methods are: the required analytical instruments are generally placed in standard analytical laboratories far away from the site, and the instruments are expensive, complicated to operate, and require the operation of professionals; sample pretreatment takes a long time, and it is difficult to meet the requirements of pesticide residues in agricultural products. On-site rapid detection needs
[0004] In the prior art, although the electrochemical sensor is convenient to carry, the quantitative detection sensitivity of pesticide residues is low, and the electrochemical sensor needs an electronic medium as a probe to indicate the strength of the electrochemical signal, and these probes will pollute the sample and affect the detection result. Interference, resulting in inaccurate test results

Method used

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  • A kind of tebuconazole molecularly imprinted membrane electrode, portable sensor and its use method and application
  • A kind of tebuconazole molecularly imprinted membrane electrode, portable sensor and its use method and application
  • A kind of tebuconazole molecularly imprinted membrane electrode, portable sensor and its use method and application

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preparation example Construction

[0048] The present invention provides a method for preparing a tebuconazole molecularly imprinted membrane electrode described in the above technical solution, comprising the following steps:

[0049] (1) placing the substrate electrode in a tetrachloroalloy acid solution, performing electrodeposition by a constant potential method, depositing gold nanoparticles on the surface of the substrate electrode, and obtaining a gold nanoparticle modified electrode;

[0050] (2) The gold nanoparticle modified electrode obtained in the step (1) is left standing in the aqueous solution of mercaptographene, so that the mercaptographene is modified on the gold nanoparticle surface of the electrode to obtain the mercaptographene-gold nanoparticle modification electrode;

[0051] (3) The mercaptographene-gold nanoparticle modified electrode obtained in the step (2) is placed in a mixed solution containing potassium nitrate, tetrachloroalloy acid and potassium ferrocyanide, and gold-nanoparti...

Embodiment 1

[0101] Soak the glassy carbon electrode in a mixed solution of 30% hydrogen peroxide and concentrated sulfuric acid with a volume ratio of 1:3 for 20 minutes, and use 0.05 μm Al 2 o 3 Grind and polish until the surface of the electrode is mirror-like, wash it with water, and ultrasonicate it in water for 10 minutes. Dry the electrode after ultrasonication with nitrogen, place it in 0.5 mol / L dilute sulfuric acid solution, and scan 20 cycles in the voltage range of -0.2 ~ 1.6V by cyclic voltammetry. After scanning, the electrode was washed with water and dried with nitrogen to obtain the pretreated substrate electrode.

[0102] Place the pretreated substrate electrode in 3mmol / L tetrachloroalloy acid solution, and use the constant potential method to electrodeposit at a voltage of -0.2V for 100s, so that gold nanoparticles are deposited on the surface of the substrate electrode to obtain a gold nanoparticle modified electrode. .

[0103] The gold nanoparticle-modified electr...

Embodiment 2

[0109] The base electrode was placed in 2.5mmol / L tetrachloroalloy acid solution, and the electrodeposition was carried out at -0.1V for 80s by the constant potential method, so that the gold nanoparticles were deposited on the surface of the base electrode, and the gold nanoparticles modified electrode was obtained.

[0110] The gold nanoparticle-modified electrode was placed in a 0.25 mg / mL mercaptographene aqueous solution, and allowed to stand for 2 hours to obtain a mercaptographene-gold nanoparticle-modified electrode.

[0111] The mercaptographene-gold nanoparticle modified electrode was placed in the mixed solution, and gold-Prussian blue was deposited by cyclic voltammetry under the conditions of potential range 0-1.0V, scanning rate 50mV / s, and scanning 15 circles to obtain gold- Prussian blue-mercaptographene-gold nanoparticles modified electrodes. The mixed solution is 0.05mol / L potassium nitrate, 0.5mmol / L tetrachloroalloy acid and 0.5mmol / L potassium ferrocyanide...

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Abstract

The invention provides a tebuconazole molecularly imprinted membrane electrode. The tebuconazole molecularly imprinted membrane electrode comprises a substrate electrode, gold nanoparticles, sulfydryl graphene, gold-prussian blue and a tebuconazole molecularly imprinted membrane, wherein the gold nanoparticles, the sulfydryl graphene and the gold-prussian blue are modified on the surface of the substrate electrode in sequence; the tebuconazole molecularly imprinted membrane is attached to the surface of the gold-prussian blue; the tebuconazole molecularly imprinted membrane is a polymer of ortho-aminophenol and m-dihydroxybenzene taking tebuconazole as a template molecule. When the tebuconazole molecularly imprinted membrane electrode provided by the invention is applied to quantitative detection, the detection limit can be up to 1.63*10<-8>mol / L, and high sensitivity is achieved. By adopting the tebuconazole molecularly imprinted membrane electrode, a probe molecule, namely, prussian blue is fixed on the tebuconazole molecularly imprinted membrane electrode, so that direct measurement of a non-electroactive target compound in a sample can be realized, and a sensor is easier and more convenient to operate, and is suitable for rapid on-site detection.

Description

technical field [0001] The invention relates to the technical field of pesticide detection, in particular to a tebuconazole molecularly imprinted membrane electrode, a portable sensor, and a use method and application thereof. Background technique [0002] Tebuconazole is a high-efficiency triazole fungicide, which is widely used in the disease control of peanuts, barley, rice, apples and other crops. Frequent use will cause soil pollution and endanger the ecosystem, groundwater and human health. [0003] At present, the method for detecting tebuconazole is mainly chromatographic technology, which has the advantages of high sensitivity, good accuracy, and excellent qualitative and quantitative analysis. However, the limitations of these analysis methods are: the required analytical instruments are generally placed in standard analytical laboratories far away from the site, and the instruments are expensive, complicated to operate, and require the operation of professionals; ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/333
CPCG01N27/30G01N27/3335
Inventor 齐沛沛王新全王祥云王娇汪志威徐霞红徐浩章虎王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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