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Rice spike length major QTLPL6-5 positioning, and molecular marker linked with major QTLPL6-5

A molecular marker, rice technology, applied in the fields of molecular biology and genetic breeding, can solve the problems of rare fine mapping, cloned ear length QTL, etc., and achieve the effect of large effect value and high stability

Active Publication Date: 2017-06-13
HUNAN HYBRID RICE RES CENT +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, most studies on panicle length QTL are still in the initial stage of mapping due to environmental and genetic factors, and there are few reports on fine mapping and cloning of panicle length QTL; those that can be stably inherited and used for molecular marker-assisted selection of rice panicle length Main effect QTL is even rarer

Method used

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  • Rice spike length major QTLPL6-5 positioning, and molecular marker linked with major QTLPL6-5
  • Rice spike length major QTLPL6-5 positioning, and molecular marker linked with major QTLPL6-5
  • Rice spike length major QTLPL6-5 positioning, and molecular marker linked with major QTLPL6-5

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Embodiment 1

[0032] A localization of the rice panicle length main effect QTL PL6-5 (also QTL PL6-5) of the present invention, the rice panicle length main effect QTL PL6-5 is located on the long arm of the rice chromosome 6, and is located on the molecular marker RM20118 and the molecular marker Between the markers RM20210, the forward primer F of the molecular marker RM20118 is shown in SEQ ID No.1, the reverse primer R of the molecular marker RM20118 is shown in SEQ ID No.2, and the forward primer F of the molecular marker RM20210 is shown in SEQ ID No. .3, the reverse primer R of molecular marker RM20210 is shown in SEQ ID No.4.

[0033] SEQ ID No.1 is: 5'-AGGCGACAGGGACACGATGG-3'

[0034]SEQ ID No.2 is: 5'-AAGCCGGATATTCCGTGTGAAGG-3'

[0035] SEQ ID No.3 is: 5'-TCGCTGTCTACTGATGATGATGG-3'

[0036] SEQ ID No.4 is: 5'-CAAAGCATATGGAAACCCTAGCC-3'

[0037] In this embodiment, there is a molecular marker M6-7 between the molecular marker RM20118 and the molecular marker RM20210, the forward...

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Abstract

The invention provides rice spike length major QTLPL6-5 positioning, and a molecular marker linked with the major QTLPL6-5. The rice spike length major QTLPL6-5 is positioned on the long arm of the chromosome 6 of rice, is positioned between a molecular marker RM20118 and a molecular marker RM20210, and is finely positioned between RM20118 and M6-7; the molecular markers RM20118, M6-7 and RM20210 are respectively positioned on the long arm of the chromosome 6 of rice; the distance from the RM20118 to the M6-7 is 1.3Mb; the distance from the M6-7 to the RM20210 is 1.2Mb. The major QTLPL6-5 provided by the invention can be stably detected in different environment and genetic backgrounds; new bit selection is provided for rice spike type improvement seed breeding; the molecular marker provides a powerful tool for fine positioning and cloning of the rice spike length QTLPL6-5.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and genetic breeding, and in particular relates to the location of the rice panicle length main effect QTLPL6-5 and the molecular marker linked with the rice panicle length main effect QTLPL6-5. Background technique [0002] The length of rice panicle plays an important role in the formation of traits such as grain density, ratio of primary and secondary branches to grain, and curvature of panicle neck, directly affects traits such as grain setting rate and number of grains per panicle, and ultimately restricts the increase of rice yield. After the variety is dwarfed, the harvest index is already very high. To further increase the rice yield, we should rely on increasing the biological yield; many indica rice breeders believe that breeding a variety or combination with a long spike and a large number of branches at a time is the solution On the basis of high biological yield, it is an ef...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 孙志忠段美娟袁定阳余东丁佳谭炎宁孙学武盛夏冰袁贵龙
Owner HUNAN HYBRID RICE RES CENT
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