A probe library, detection method and kit for detecting met gene exon 14 mutation

Abstract

The invention provides a probe for detecting MET gene 14 exon mutation and a detection method. A DNA probe library comprises one or more DNA probes capable of hybridizing with an MET gene 14 exon and adjacent introns. The DNA probes are as shown in SEQ ID NO.1 to No.46. In addition, the invention provides a method for performing enrichment and detection on the MET gene 14 exon and the adjacent introns by using the probe library. The method is combined with a next-generation sequencing (NGS) technology, so the sensitivity, the accuracy and the comprehensiveness of MET14 gene detection can be greatly improved.

Description

technical field [0001] The invention relates to the field of gene detection, in particular, the invention relates to a probe and a detection method for detecting the mutation of exon 14 of the MET gene. This method can accurately enrich the mutation sequence of exon 14 of the MET gene, and the obtained DNA sample library can be further combined with next-generation sequencing (NGS) to accurately determine the mutation of exon 14 of the MET gene in patients, and provide information for related tumors. Provide guidance and theoretical basis for diagnosis, prognosis, and design of clinical treatment plans. Background technique [0002] Primary lung cancer (hereinafter referred to as lung cancer) occurs in the epithelium of the bronchial mucosa, so it is also called bronchial lung cancer. It is the most common primary malignant tumor of the lung and one of the most common malignant tumors in my country. healthy. [0003] In my country, the morbidity and mortality of lung cancer...

AI Technical Summary

Problems solved by technology

Its disadvantages include: it is a single-base mutation detection, it cannot quantify mRNA, and cannot detect gene transversions; it has low sensitivity; it takes a long time, and only one gene mutation can be detected at a time; it cannot determine the specific changes of bases; Perform high-throughput assays

Its disadvantages include: it cannot detect single-base type mutations; it cannot detect gene transversions; it can only detect known mutations; it takes a long time, and only one gene mutation can be detected at a time; it cannot determine the specific changes of bases

Its disadvantages include: it cannot detect gene transversions; it can only detect known mutations; it has high cost and low throughput; it has low accuracy and usually needs to be repeated more than 2 times to confirm the results

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