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Bacillus cereus solid fermentation method, as well as fermented product and application thereof

A Bacillus cereus, solid fermentation technology, applied in the direction of using spores, applications, botanical equipment and methods, etc., can solve the problem of death, reduce the activity and quality stability of the inoculum, the number of viable bacteria and the low spore rate, etc. problems, to achieve the effect of promoting crop growth, good application prospects, and short fermentation cycle

Inactive Publication Date: 2017-06-13
山东五福生生态工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the fermentation method of Bacillus cereus is mainly liquid fermentation. This fermentation method generally has the problem of low number of viable bacteria and low spore rate. Bacteria without spores will die in a short time, which greatly reduces the activity and Quality stability

Method used

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  • Bacillus cereus solid fermentation method, as well as fermented product and application thereof
  • Bacillus cereus solid fermentation method, as well as fermented product and application thereof

Examples

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Effect test

Embodiment 1

[0099] A kind of bacillus cereus solid fermentation method, comprises the following steps:

[0100] (a) slant seed activation: inoculate the Bacillus cereus strain on a nutrient agar slant medium sterilized at 121° C. and 0.11 MPa for 20 minutes under aseptic conditions, cultivate at a temperature of 37° C., and cultivate for 36 hours to obtain slant seeds;

[0101] The components of the nutrient agar slant medium include 3g / L beef extract, 10g / L peptone, 5g / L sodium chloride and 20g / L agar, and the pH value is 7.0;

[0102] (b) Liquid seed culture: pick two rings of slant seeds obtained in step (a) and inoculate them in a liquid medium sterilized at 121°C and 0.11MPa for 30 minutes in a 1000L liquid fermenter, culture temperature is 37°C, and culture time is 36h , get liquid seeds;

[0103] The components of the liquid medium include 3g / L beef extract, 10g / L peptone and 5g / L sodium chloride, and the pH value is 7.0;

[0104] (c) Solid fermentation culture: put the component...

Embodiment 2

[0108] A kind of bacillus cereus solid fermentation method, comprises the following steps:

[0109] (a) slant seed activation: inoculate the Bacillus cereus strain on a nutrient agar slant medium sterilized at 121° C. and 0.11 MPa for 60 minutes under aseptic conditions, cultivate at a temperature of 36° C., and cultivate for 40 hours to obtain slant seeds;

[0110] The components of the nutrient agar slant medium include 3g / L beef extract, 10g / L peptone, 5g / L sodium chloride and 20g / L agar, and the pH value is 7.2;

[0111] (b) Liquid seed culture: Pick two rings of slant seeds obtained in step (a) and inoculate them in a liquid medium sterilized at 121°C and 0.11MPa for 60 minutes in a 1000L liquid fermenter, at a culture temperature of 36°C and for a culture time of 48 hours , get liquid seeds;

[0112] The components of the liquid medium include 3g / L beef extract, 10g / L peptone and 5g / L sodium chloride, and the pH value is 7.2;

[0113] (c) Solid fermentation culture: pu...

Embodiment 3

[0117] A kind of bacillus cereus solid fermentation method, comprises the following steps:

[0118] (a) slant seed activation: inoculate the Bacillus cereus strain on the nutrient agar slant medium sterilized at 121° C. and 0.11 MPa for 30 minutes under aseptic conditions, cultivate at 38° C. and cultivate for 48 hours to obtain slant seeds;

[0119] The components of the nutrient agar slant medium include 3g / L beef extract, 10g / L peptone, 5g / L sodium chloride and 20g / L agar, and the pH value is 7.1;

[0120] (b) Liquid seed culture: Pick two rings of slant seeds obtained in step (a) and inoculate them in a liquid medium sterilized at 121°C and 0.11MPa for 40 minutes in a 1000L liquid fermenter, culture temperature is 38°C, and culture time is 40 hours , get liquid seeds;

[0121] The components of the liquid medium include 3g / L beef extract, 10g / L peptone and 5g / L sodium chloride, and the pH value is 7.1;

[0122] (c) Solid fermentation culture: put the components of the so...

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Abstract

The invention provides a bacillus cereus solid fermentation method, as well as a fermented product and application thereof, and relates to the technical field of microbial fermentation. The fermentation method comprises the following steps: after seed activation and liquid seed cultivation are performed on a bacillus cereus strain, a solid culture medium is adopted to carry out fermentation cultivation, and the fermented product is obtained; wherein the solid culture medium is prepared from the following components in parts by weight: 40 to 60 parts of mushroom residues, 10 to 30 parts of rice husks, 15 to 35 parts of bran and 2 to 8 parts of corn syrup, and the pH value of the solid culture medium is 6.5 to 7.5. According to the bacillus cereus solid fermentation method, the problem that the number of viable bacteria and the sporation rate are relatively low as the conventional bacillus cereus fermentation mode adopts liquid fermentation is relieved; by the adoption of a high-density solid fermentation method for producing the bacillus cereus, the process is simple and easy to implement; due to the method, growth of the bacillus cereus can be obviously promoted, and the number of viable bacillus cereus and the sporation rate are increased.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a solid fermentation method of Bacillus cereus, fermentation products and applications thereof. Background technique [0002] Bacillus cereus is a species of Bacillus. Bacteria cells are rod-shaped, end square, in short or long chains, 1.0-1.2×3.0-5.0 microns, spore-forming, spores are round or columnar, mesozoic or nearly mesozoic, 1.0-1.5 microns, no obvious cysts Swelling, Gram-positive, acapsular, motile, large colonies, surface rough, flat, irregular. Colony morphology of Bacillus cereus: On ordinary agar plate medium, cultured at 37°C for 24 hours, a round or nearly round, soft, colorless, slightly glossy white colony with a diameter of 5-7mm can be formed. Bacillus cereus grows more vigorously on the M.S.P medium. The diameter of the colony reaches 8-10mm, the texture is softer, and it is filamentous when picked up. The culture time is slightly longer. The...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N3/00A01N63/00A01P5/00A01P21/00C12R1/085
CPCC12N1/20A01N63/00C12N3/00
Inventor 刘峰王江伟潘明王传磊王珍伟张鲁新王培朱洪菊
Owner 山东五福生生态工程有限公司
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