Mitochondria-target fluorescent carbon dot as well as preparation method and application thereof

Abstract

The invention discloses a mitochondria-target fluorescent carbon dot as well as a preparation method and application thereof. The fluorescent carbon dot is mainly prepared from the following raw materials in parts by weight: 1-20 parts of chitosan, 1-10 parts of ethylenediamine and 1-10 parts of mercaptobutanedioic acid; and the carbon dot with excellent fluorescence property is prepared from the chitosan, the ethylenediamine and the mercaptobutanedioic acid as raw materials through a hydrothermal method in one step. Compared with the prior art, the carbon dot is capable of achieving fast, no-clean and long-term photostable (anti-bleaching) imaging on mammalian cell mitochondria, and can be used for directionally conveying an anti-tumor drug to the mitochondria as a carrier, thereby achieving mitochondria-based targeted cancer therapy. In addition, the carbon dot also has the advantages of low preparation cost, good water dispersibility and low cytotoxicity, and hopefully replaces a commercial mitochondria fluorescent probe widely used at present.

Description

technical field [0001] The invention discloses a mitochondria-targeted fluorescent carbon point, a preparation method and an application thereof, and belongs to the technical field of bionano materials. Background technique [0002] Whether in mammalian normal cells or diseased cells, mitochondria have a profound impact on the life activities of cells with their unique functions, such as ATP production and cell energy supply, calcium ion signals inside cells and the generation of free radicals, The regulation of apoptosis and the maintenance of the homeostasis of the internal environment of cells, etc. Since mitochondria are of great significance to cell metabolism, the dysfunction of mitochondria is closely related to the occurrence and development of diseases such as diabetes, tumors, Parkinson's disease and Alzheimer's disease. Therefore, the observation and research of the number and shape of mitochondria are very important for detecting the state and life activities of...

AI Technical Summary

Problems solved by technology

However, since the ligand is highly toxic and does not have fluorescent properties, it is usually necessary to introduce other fluorescent molecules during the modification process to realize the fluorescent tracking of the drug, but the introduction of other molecules will reduce the concentration of the modified drug. Mitochondrial targeting

These shortcomings have also become the bottleneck for the ligand to achieve mitochondrial targeted delivery of drugs

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