Control method of solanaceous vegetable soil continuous cropping obstacles
A technology of soil continuous cropping obstacles and solanaceous fruits, which is applied in the agricultural field, can solve the problems of general control effects, etc., and achieve the effects of sufficient nutrition, strong root growth, and increased soil organic matter content
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Embodiment 1
[0028] Preparation of biological bacterial fertilizer for preventing continuous cropping obstacles in solanaceous soil:
[0029] An antagonistic bacteria BZ6-1 isolated in the laboratory is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens), which was preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms on January 22, 2009, with a preservation number of CGMCC NO.2892, and in China Fully disclosed in patent application 201010217803.4. A growth-promoting bacterium SC-12 is Bacillus stratosphericus, which was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 15, 2013. The preservation number is CGMCC NO.7622, and it is patented in China. Fully disclosed in application 201310646842.X.
[0030] The antagonistic bacteria BZ6-1 and growth-promoting bacteria SC-12 were inoculated into LB liquid medium respectively for liquid fermentation production. Fermentati...
Embodiment 2
[0034] Example 2 Effects of different lime nitrogen application rates and control methods on the physical and chemical properties of pepper continuous cropping soil
[0035] Table 2 shows the effects of different lime nitrogen application rates and control methods on the physical and chemical properties of pepper continuous cropping soil. It can be seen from Table 2 that the application of lime nitrogen can increase the soil pH of continuous cropping, from 5.4 to 6.85, and it increases with the increase of the application amount of lime nitrogen. This is because lime nitrogen contains about 60% lime, which can increase the soil pH when applied to the soil. Application of chemical fertilizers can accelerate soil acidification. It can also be seen from Table 2 that compared with individual lime nitrogen application and lime nitrogen and chemical fertilizer treatment, lime nitrogen disinfection and biological bacterial fertilizer treatment can significantly improve the organic m...
Embodiment 3
[0038] Embodiment 3 urease activity assay
[0039] Take 2g of air-dried soil, put it into a 50mL Erlenmeyer flask, and add 0.5mL of toluene. After 15 min, 5 mL of 10% urea solution and 10 mL of citrate buffer at pH 6.7 were added. After shaking well, put it into a 37°C incubator and incubate for 24h. Filter, then take 1mL of the filtrate and pour it into a 50mL volumetric flask, then add 4mL of sodium phenoxide solution and 3mL of sodium hypochlorite solution, shake well, and start to develop color after 20 minutes. Standard curve drawing: draw the diluted standard solution 1, 3, 5, 7, 9, 11, 13mL and dilute to 20mL, then add 4mL sodium phenoxide solution and 3mL sodium hypochlorite solution, shake well, start to develop color after 20 minutes, then Volume. Colorimetric at 578nm within 1h. And draw a standard curve. NH produced by 1 g of soil after 24 hours for urease activity 3 The mg number of -N means: NH 3 -N(mg)=a*2 In the formula, a-NH found from the standard curv...
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