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Experimental method for proving connected relation of estrogen and gap

A gap junction and estrogen technology, which is applied in the field of experiments to prove the relationship between estrogen and gap junction, can solve the problem that the gap junction relationship has not been elucidated.

Inactive Publication Date: 2017-05-31
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, in the prior art, there are no related reports that elucidate the effect of estrogen on improving the inflammatory response leading to hypertension and its relationship with gap junctions

Method used

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  • Experimental method for proving connected relation of estrogen and gap
  • Experimental method for proving connected relation of estrogen and gap
  • Experimental method for proving connected relation of estrogen and gap

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Experimental program
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Embodiment Construction

[0021] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0022] grouping:

[0023] WKY group: Normal Wistar Kyoto (Wistar-Kyoto, WKY) rats, male or female, 10 weeks old, were intraperitoneally injected with the same amount of normal saline as the estrogen intervention group for 4 weeks.

[0024] SHR group: spontaneously hypertensive rats (spontaneously hypertensive rats, SHR), male or female, 10 weeks old, intraperitoneally injected with the same amount of normal saline as estrogen intervention group, for 4 weeks.

[0025] Estrogen intervention group: SHR, male or female, 10 weeks old, received intraperitoneal injection of 20 μg / kg / day body weight of estrogen daily for 4 weeks.

[0026] 1 Animal blood pressure measurement Use a fully automatic non-invasive blood pressure measuring instrument for rats and mice to detect the systolic pressure of the tail artery of W...

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Abstract

The invention discloses an experimental method for proving connected relation of estrogen and gap. The method comprises the following steps: measuring animal blood pressure; taking out rat kidney by HE dyeing of kidney tissues; preparing rat peripheral blood lymphocyte suspension; and detecting peripheral blood T lymphocytal subset and Cx0 / Cx43.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an experimental method for proving the relationship between estrogen and gap junctions. Background technique [0002] Gap junction, also known as communication junction, is a form of cell connection that exists widely in other tissue cells except for skeletal muscle cells, and it is a patchy scattered local connection. Under the electron microscope, the adjacent cell membranes at the junction are highly parallel, the cell gap is very narrow, only 2-3nm, and there are many observation points with approximately equal intervals. Observation by cryo-etching electron microscope shows that there are many regularly arranged columnar particles on the adjacent cell membrane, which are called connexons. Each connectosome is composed of 6 rod-shaped connexins, and the center is surrounded by a hydrophilic tubule with a diameter of about 2 nm, called the central canaliculum. The con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
CPCG01N15/14
Inventor 李新芝马克涛倪欣
Owner SHIHEZI UNIVERSITY
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