Silkworm silk fibroin nanofiber and preparation method thereof

A silk fibroin and nanofiber technology, applied in spinning solution preparation, single-component silk fibroin man-made filament, fiber processing, etc. and other problems, to achieve the effect of reducing production cost, increasing length, and shortening the process

Active Publication Date: 2017-05-31
NANTONG TEXTILE & SILK IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese invention patents "a silk fibroin nanofiber membrane and its preparation method" (103341209A) and "a device for preparing functional nanofibers" (105734697A) are prepared by conventional or membrane-split electrospinning methods. Fiber requires high energy consumption and special large-scale equipment, and the production cost is relatively high, and the prepared nanofibers are deposited on a two-dimensional plane, which is difficult to meet the requirements of three-dimensional use; Chinese invention patent "a linear nano-hydroxyapatite Preparation method of "(102491298B) and "A non-crystalline silk fibroin nanofiber solution and its preparation method" (105968384A) etc. to prepare nanofibers by inducing and cultivating self-assembly method, although no fixed large-scale equipment is required, but the prepared The length of nanofibers is short and cannot reach the micron level, which is difficult to meet the actual use requirements

Method used

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  • Silkworm silk fibroin nanofiber and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This embodiment provides a method for preparing silk fibroin nanofibers, the specific steps are as follows:

[0023] (1) Dissolve and dialyze the degummed and dried pure silk fibroin fibers with 9.3M lithium bromide to obtain a fresh silkworm silk fibroin solution, which is poured into a polystyrene mold with a thickness of 1 mm. Rapid drying and molding under % humidity environment.

[0024] (2) Soak the silk fibroin film formed after rapid drying in ethanol for 30 minutes at room temperature to obtain a recrystallized silk fibroin film.

[0025] (3) Soak the recrystallized silk fibroin film at a ratio of 1 / 30 (w / v) in tris-hydrochloric acid buffer solution at 37°C, 0.001M, pH=7.0 for 0.5h, and then Centrifuge at 5000 rpm for 14 min to separate the supernatant.

[0026] (4) After diluting the supernatant obtained above to 0.5 mg / ml, culture in a constant temperature water bath environment for 3 hours to obtain silk fibroin nanofiber aggregates in a wet state.

[002...

Embodiment 2

[0030] (1) Dissolve and dialyze the degummed and dried pure silk fibroin fibers with 9.3M lithium bromide to obtain a fresh silkworm silk fibroin solution, which is poured into a polystyrene mold with a thickness of 3 mm, at 25°C, 60 Rapid drying and molding under % humidity environment.

[0031] (2) Soak the silk fibroin film formed after rapid drying in ethanol for 60 minutes at room temperature to obtain a recrystallized silk fibroin film.

[0032] (3) Soak the recrystallized silk fibroin film at a ratio of 1 / 50 (w / v) in 37°C, 0.01M, pH=7.0 sodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution, soaking time is 1 h, then centrifuged at 3000 rpm for 17 min, and separated to obtain the supernatant.

[0033] (4) After diluting the supernatant obtained above to 0.25 mg / ml, culture in a constant temperature water bath environment for 6 hours to obtain silk fibroin nanofiber aggregates in a wet state.

[0034] (5) Freeze-drying the above aggregates of wet silk fi...

Embodiment 3

[0036] (1) Dissolve and dialyze the degummed and dried pure silk fibroin fibers with 9.3M lithium bromide to obtain a fresh silkworm silk fibroin solution, which is poured into a polystyrene mold with a thickness of 2 mm. Rapid drying and molding under % humidity environment.

[0037] (2) Soak the silk fibroin film formed after rapid drying in ethanol for 120 minutes at room temperature to obtain a recrystallized silk fibroin film.

[0038] (3) Soak the recrystallized silk fibroin film at a ratio of 1 / 30 (w / v) in tris-hydrochloric acid buffer solution at 37°C, 0.05M, pH=7.0 for 0.5h, then 7000 Centrifuge at rpm for 10 min and separate to obtain the supernatant.

[0039] (4) After diluting the supernatant obtained above to 0.75 mg / ml, culture in a constant temperature water bath environment for 3 hours to obtain silk fibroin nanofiber aggregates in a wet state.

[0040] (5) Freeze-drying the above aggregates of wet silk fibroin nanofibers to obtain dry silk fibroin nanofibers...

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Abstract

The invention discloses silkworm silk fibroin nanofiber and a preparation method thereof. Silkworm silk is used as a raw material, hydrophilic silkworm silk fibroin peptide chains are obtained through dissolution, regeneration and extraction, self-assembly is performed in a mild water phase environment, and the nanofiber with the diameter of 20-500 nm and the length up to 1-100 mu m is obtained. During preparation of the silkworm silk fibroin nanofiber, the nanofiber in a larger size can be obtained through self-assembly with no additive required, the method has the characteristics that the process is simple, the condition is mild and the production cost is low, and the prepared nanofiber can meet the actual use requirement.

Description

technical field [0001] The invention relates to a nanofiber, in particular to a nanofiber prepared from silkworm silk fibroin and a preparation method thereof. Background technique [0002] Natural silk fibroin fiber is favored for its excellent tensile strength and ductility, and is widely used in light industry, medical treatment and other fields. Regenerated Bombyx mori silk fibroin is widely used in tissue repair, drug controlled release, Research reports in the field of wound accessories and cosmetology are not uncommon. However, whether it is regenerated silk fibroin film, porous scaffold, gel and other systems, it is difficult to match the excellent physical and mechanical properties of natural silk fibroin fibers. The regenerated silk fibroin system is often microscopically isotropic, which is difficult to meet the requirements of anisotropic cell culture and reinforcement of regenerated silk fibroin substrate. [0003] Studies have found that the excellent physic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01D5/42D01D1/02D01F4/02
CPCD01D1/02D01D5/423D01F4/02
Inventor 殷祝平卢神州吴锋邢铁玲
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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