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Preparation method of micafungin parent nucleus FR179642

A technology of micafungin and mother nucleus, which is applied in the field of biopharmaceuticals, can solve the problems of high preparation costs, achieve good reproducibility, simple operation procedures, and increase production

Inactive Publication Date: 2017-05-31
WUXI FORTUNE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the above-mentioned prior art has the defect that the preparation cost is high
At the same time, there is no relevant report on the fermentation preparation of FR179642 in the prior art, which belongs to the current blank technology in the industry

Method used

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  • Preparation method of micafungin parent nucleus FR179642
  • Preparation method of micafungin parent nucleus FR179642

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the micafungin parent nucleus FR179642 includes the following steps.

[0030] Step (1), take a small amount of micafungin parent nucleus FR179642 to produce a bacterial spore suspension, inoculate it on the seed medium, and cultivate at 24° C. for 50 hours. The components in the seed medium are (unit: g / L): glucose 20, yeast powder 5, soybean meal powder 5, dipotassium hydrogen phosphate 0.5, sodium chloride 0.1, magnesium sulfate 0.1, calcium carbonate 0.1, the remainder for water.

[0031] In step (2), the seed medium is inoculated into the fermentor, and the fermentation medium is added, and cultured at 24 to 28° C. for 60 to 70 hours. When the bacterial concentration in the fermenter reached a steady state, the precursor of micafungin FR901379 was added, and after the fermentation was continued for 40 h, the precursor of micafungin FR901379 was added. In these two fermentation stages, from the initial state to the first fermentation stage...

Embodiment 2

[0037] The preparation method of the micafungin parent nucleus FR179642 includes the following steps.

[0038] Step (1), take a small amount of micafungin parent nucleus FR179642 to produce a bacterial spore suspension, inoculate it on the seed medium, and cultivate at 28° C. for 40 hours. The components in the seed medium are (unit: g / L): glucose 35, yeast powder 15, soybean meal powder 15, dipotassium hydrogen phosphate 1.5, sodium chloride 1.0, magnesium sulfate 1.0, calcium carbonate 0.5, the remainder for water.

[0039] In step (2), the seed medium is inoculated into the fermentor, and the fermentation medium is added, and cultured at 24 to 28° C. for 60 to 70 hours.

[0040]When the bacterial concentration in the fermenter reaches a steady state, the precursor of micafungin FR901379 is added, and after the fermentation is continued for 60 hours, the precursor of micafungin FR901379 is added. In these two fermentation stages, from the initial state to the first ferment...

Embodiment 3

[0046] The preparation method of the micafungin parent nucleus FR179642 includes the following steps.

[0047] Step (1), take a small amount of micafungin parent nucleus FR179642 to produce a bacterial spore suspension, inoculate it on the medium, and cultivate at 26° C. for 48 hours. The components in the seed medium are (unit: g / L): glucose 25, yeast powder 10, soybean meal powder 10, dipotassium hydrogen phosphate 1, sodium chloride 0.5, magnesium sulfate 0.5, calcium carbonate 0.2, the balance for water.

[0048] In step (2), the seed medium is inoculated into the fermentor, and the fermentation medium is added, and cultured at 24 to 28° C. for 60 to 70 hours.

[0049] Micafungin precursor FR901379 was added when the bacterial concentration in the fermenter reached a steady state. After the fermentation was continued for 50h, the precursor of micafungin, FR901379, was added. In these two fermentation stages, from the initial state to the first fermentation stage when th...

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Abstract

The invention provides a micafungin parent nucleus producing bacterium strain and a method for producing micafungin parent nucleus by adding a micafungin precursor and carrying out deacylation on the micafungin precursor in the fermentation process, belonging to the field of biopharmacy. The method comprises the following steps: fermenting a micafungin parent nucleus FR179642 strain in a culture medium, wherein a micafungin precursor FR901379 is added twice in the fermentation process, and a multistage temperature control process is adopted, thereby enhancing the yield of the micafungin parent nucleus FR179642; and carrying out acidifying wall breaking treatment on the fermentation liquid, extracting with an organic solvent, carrying out macroporous resin adsorption-desorption to obtain a desorption solution, and crystallizing the desorption solution to obtain the micafungin parent nucleus powder. The method can be used for synthesizing the micafungin parent nucleus FR179642, and has the advantages of simple operating procedure, high stability and favorable reproducibility.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a preparation method of micafungin parent nucleus FR179642. Background technique [0002] In recent years, with the large-scale application of broad-spectrum antibiotics and immunosuppressants, tumor chemotherapy, organ transplantation, and the spread of AIDS, the number of people with suppressed immune systems continues to increase, and the incidence of deep fungal infections has begun to show a clear upward trend. , and with the use of antifungal drugs, the drug resistance of fungi is becoming stronger and stronger, which makes the application of antifungal drugs have a trend of rapid increase. Therefore, anti-deep fungal infection drugs have become one of the research hotspots of anti-infective drugs, and are increasingly attracting people's attention. [0003] Echinocandins are a new type of antifungal drug, which is obviously different from the current clinical a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C07K1/30C07K1/16C07K1/14
CPCC07K7/56
Inventor 游云龙付静曹峥杨亮赵永俊蔡云洁金丹甜
Owner WUXI FORTUNE PHARMA
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