Microbial preparation for sludge anaerobic fermentation

A technology of microbial preparations and mixed bacterial solutions, applied in the direction of microorganism-based methods, microorganisms, sludge treatment, etc., can solve the problems of reduced methane production efficiency and waste of resources, and achieve reduced treatment costs, improved efficiency, and improved nitrogen removal performance Effect

Inactive Publication Date: 2017-05-31
王筠涵
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] In the anaerobic digestion process of sludge, although it mainly relies on concentration and dehydration, the organic matter is anaerobically decomposed and converted into biogas, which itself is also a digestion process. In the prior art, the process of generating biogas from sludge mainly relies on methane production Bacteria consume formic acid, acetic acid and methanol, and organic acid substances are produced by digestion and decomposition of organic matter by enzymes such as cellulase, protease and lipase, and then remove formic acid, acetic acid and methanol that can be directly used by methanogenic bacteria , other acids and alcohols, such as propionic acid and butyric acid, cannot be used as substrates for methanogenic bacteria to produce methane, and cannot be decomposed and utilized by methanogenic bacteria, which largely causes waste of resources and greatly reduces the efficiency of methane production

Method used

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  • Microbial preparation for sludge anaerobic fermentation

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Experimental program
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Effect test

Embodiment 1

[0033] A microbial preparation is prepared, and the microbial preparation is composed of a mixed bacterial solution and a carrier, and the mixed bacterial solution is prepared from Bacillus pumilus, Enterobacter cloacae, denitrifying bacteria, Paracoccus denitrificans, Bacillus amyloliquefaciens and Lactococcus lactis.

[0034] Described bacillus pumilus is (Bacillus pumilus) ATCC27142;

[0035] The Enterobacter cloacae is ( Enterobacter cloacae ) ATCC 700323;

[0036] The denitrifying bacteria is (Paracoccus pantotrophus) ATCC 35512;

[0037] The paracoccus denitrificans is (Paracoccus denitrificans) ATCC13543;

[0038] The bacillus amyloliquefaciens is ( Bacillus amyloliquefaciens ) ATCC 23843;

[0039]The Lactococcus lactis (Lactococcus lactis) ATCC 11454.

[0040] The carrier is formed by mixing kaolin and chitosan in a weight ratio of 1:3;

[0041] The microbial preparation is prepared by mixing the mixed bacterial liquid and the carrier according to the weight rat...

Embodiment 2

[0046] The sludge samples come from a sewage treatment plant and are processed according to the following steps:

[0047] 1) Adjust the sludge so that the total solid content is 8wt%;

[0048] 2) Add the sludge with a total solid content of 8% obtained in the first step into the anaerobic digestion tank, the amount added is 60% (v / v) of the volume of the digestion tank, and 8% (v / v) of the volume of the digestion tank is added ), add water to 90% of the volume of the digestion tank, and adjust the pH to 7.5-7.7;

[0049] The methanogenic inoculum is biogas fermentation liquid;

[0050] 3) Inoculate microbial preparations, the amount added is 1% (v / v) of the volume of the digestion tank, stir evenly, and cover;

[0051] 4) Keep the reaction temperature at 30°C, stir once every 10 hours, add rare earth nitrate while stirring, and react for 36 hours;

[0052] The addition amount of the rare earth nitrate is 3 μg of rare earth nitrate per gram of volatile solids (VSS) in the sl...

Embodiment 3

[0058] Example 3 Influence of Bacteria Composition on Methane Increment Experiment

[0059] Firstly, take 200kg of sludge from a sewage treatment plant and divide it into five equal parts, each of which is 40kg.

[0060] Five equally divided sludges were placed in five identical airtight reaction containers, respectively marked as the experimental group, control group 1, control group 2, control group 3, and blank control group.

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Abstract

The present invention belongs to the field of microbial technology and discloses a microbial preparation for sludge anaerobic fermentation. The microbial preparation comprises a mixed bacteria liquid and a carrier, wherein the mixed bacteria liquid is prepared from Bacillus pumilus, enterobacter cloacae, denitrifying bacteria, paracoccus denitrificans, bacillus amyloliquefaciens, and lactococcus lactis. The microbial preparation can greatly increase the amount of methane generated by sludge, increase the utilization rate of the sludge, and recycle the sludge.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a microbial preparation for anaerobic fermentation of sludge. Background technique [0002] With the acceleration of my country's urbanization process and small town construction and population increase, the public's requirements for environmental quality are increasing day by day. A large number of sewage treatment plants have been built and put into use, and the amount of sludge that follows is also increasing. Sludge is the product of sewage treatment. It is an extremely complex heterogeneous body composed of organic debris, bacterial cells, inorganic particles, colloids, etc. The main characteristics of sludge are high water content, high organic matter content, and easy It is rotten and smelly, and the particles are finer, the specific gravity is smaller, and it is in a colloidal liquid state. It is a thick substance between liquid and solid and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F11/04C12R1/07C12R1/01
CPCC02F11/04C12N1/20Y02W10/20
Inventor 王筠涵王化国
Owner 王筠涵
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