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Culture medium for Erysipelothrix rhusiopathiae

A technology of Erysipelothrix rhusiopathiae and culture medium, which is applied in the field of microbiology, can solve the problems of slow proliferation of Erysipelothrix rhusiopathiae, achieve the effect of not being easily infected with bacteria and increasing the proliferation rate

Inactive Publication Date: 2017-05-31
鼎正生物药业(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a culture medium for Erysipelothrix rhusiopathiae, so as to solve the technical problem of slow proliferation of Erysipelothrix rhusiopathiae in the prior art

Method used

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Examples

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Effect test

Embodiment 1

[0016] A culture medium for Erysipelothrix rhusiopathiae, which consists of the following ingredients in parts by weight: 8 parts of kujianpi, 6 parts of subflour powder, 2 parts of beef extract, 1 part of maltose, 1 part of Angelica dahurica, 1 part of black soybean powder, serum 1 part, 1 part cortex, 1 part rehmannia, 1 part nutmeg. The medium is a liquid medium with a pH of 7.

Embodiment 2

[0018] A culture medium for Erysipelothrix rhusiopathiae, which consists of the following ingredients in parts by weight: 8 parts of kujianpi, 6 parts of subflour powder, 2 parts of beef extract, 1 part of maltose, 1 part of Angelica dahurica, 1 part of black soybean powder, serum 1 part cork, 1 part nutmeg. The pH of the medium was 6.

Embodiment 3

[0020] A culture medium for Erysipelothrix rhusiopathiae, which consists of the following ingredients in parts by weight: 8 parts of kujianpi, 6 parts of subflour powder, 2 parts of beef extract, 1 part of maltose, 1 part of Angelica dahurica, 1 part of black soybean powder, serum 1 part, 1 part of Rehmannia glutinosa. The medium is a liquid medium with a pH of 8.

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PUM

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Abstract

The present invention provides a culture medium for Erysipelothrix rhusiopathiae, wherein the culture medium comprises, by weight, 8 parts of cortex meliae, 6 parts of wheat-middlings, 2 parts of beef extract, 1 part of maltose, 1 part of angelica dahurica, 1 part of black bean powder, and 1 part of serum, and on the basis, a specific amount of cortex phellodendri, rehmannia glutinosa or myristica fragrans houtt is further added. According to the present invention, a certain Chinese herb component is added on the basis of the commonly used materials of the culture medium, such that the unexpected prominent culture effect is obtained, and the obtained culture medium is especially suitable for the Erysipelothrix rhusiopathiae culture, and has god application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a culture medium for Erysipelothrix rhusiopathiae. Background technique [0002] Erysipelothrix erysipelas, commonly known as Erysipelothrix, belongs to the genus Erysipelas, and is a slender bacillus that appears singly, in pairs, or in small clusters in tissue contacts or blood sheets of infected animals. The strain is non-motile, non-spore-producing, and non-capsulated. The strain is relatively resistant to natural factors such as salting, smoking, drying, spoilage and sunlight. Less resistant to heat. Erysipelothrix rhusiopathiae is an important pathogen causing swine erysipelas. [0003] The in vitro culture of Erysipelothrix rhusiopathiae is an important basis for studying its physiological characteristics. In the prior art, the in vitro culture medium for Erysipelothrix rhusiopathiae has deficiencies in terms of growth rate and biomass accumulation. Contents of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 刘鼎阔王立红张凤洪张俊霞
Owner 鼎正生物药业(天津)有限公司
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