Strain for generating pullulan and method for producing pullulan by fermenting strain

A technology of pullulan polysaccharide and bacterial strain, applied in the field of fermentation and production of pullulan polysaccharide, can solve problems such as difficulty, difficult separation, increase of separation steps and cost, etc., and achieve simple raw materials, simple and easy method, and simplified post-processing technology Effect

Inactive Publication Date: 2017-05-31
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because Aureobasidium pullulan produces melanin during the synthesis of pullulan, the melanin will be adsorbed on the pullulan molecule, which is not easy to separate, which will cause difficulties in the downstream processing of pullulan and increase the separation steps and costs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Take a ring of Aureobasidium pullulans HL-1 slant strain cultured at 26°C for 48 hours, and inoculate it into a liquid seed medium, the liquid volume is 60ml of medium in a 500ml Erlenmeyer flask, the rotation speed is 220rpm, and cultivated at 25°C for 48h to obtain seeds liquid.

[0025] 2) Inoculate the prepared seed solution into the fermentation medium at a ratio of 10%, and put 60ml of the medium in the Erlenmeyer shaker flask with a liquid volume of 500ml, at a rotation speed of 220rpm, at 28°C, and cultivate for 60h;

[0026] The above seed medium is: 50g / l sucrose, 5g / l yeast extract, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O;

[0027] The above fermentation medium: 74g / l glucose, 2g / l sodium nitrate, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O.

[0028] 3) Centrifuge the fermented liquid obtained in step 2) to remove the bacteria, add 2 times the volume of methanol to the filtered supernatant, stir gently, let it s...

Embodiment 2

[0030] 1) Take a ring of Aureobasidium pullulans HL-1 slant strain cultured at 25°C for 48 hours, and inoculate it into a liquid seed medium, with a liquid volume of 50ml of medium in a 500ml Erlenmeyer flask, at a rotation speed of 220rpm, and cultivate at 26°C for 55h to obtain seeds liquid.

[0031] 2) Inoculate the prepared seed solution into the fermentation medium at a ratio of 10%, put 60ml of the medium in a 500ml Erlenmeyer shaker flask with a rotating speed of 220rpm, and cultivate for 60 hours at 27°C;

[0032] The above seed medium is: 50g / l sucrose, 5g / l yeast extract, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O;

[0033] The above fermentation medium: 100g / l sucrose, 2g / l sodium nitrate, 1g / l sodium chloride, 5g / l KH 2 PO 4 , 0.5g / lMgSO 4 ·7H 2 O.

[0034] 3) Centrifuge the fermented liquid obtained in step 2) to remove the bacteria, add 2 times the volume of methanol to the filtered supernatant, stir gently, let it stand for 1 hour, cent...

Embodiment 3

[0036] 1) with embodiment 1.

[0037] 2) with embodiment 1, difference is: fermentation medium: 70g / l glucose, 1.5g / l NaNO 3 , 0.5g / l NaCl, 4g / l KH 2 PO 4 , 0.1g / l MgSO 4 ·7H 2 O.

[0038] 3) with embodiment 1.

[0039] After the product was dried, the yield could reach 59.03 g / l.

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PUM

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Abstract

The invention discloses a strain for generating pullulan. The strain is a strain which is obtained from plant leaves and does not generate melanin. The classification and nomenclature of the strain is Aureobasidium pullulans HL-1 as a Latin name. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.10772. A method for producing the pullulan by fermenting the strain is simple and feasible; a product is high in conversion rate so as to facilitate later-stage separation and purification.

Description

technical field [0001] The invention relates to a high-yielding pullulan strain and a method for fermenting and producing pullulan using the strain. Background technique [0002] As an extracellular polymer synthesized by microorganisms, pullulan is easily soluble in water, safe, non-toxic, edible, and degradable. It is a multifunctional green polymer material. Its structure is composed of glucose combined into maltotriose by α-1, 4 glycosidic bonds, and the two ends are combined with other maltotriose by α-1, 6 glycosidic bonds, and so on. This unique way of connecting monomers makes it have material properties that other polysaccharides do not have: excellent plasticity, film-forming and fiber-forming capabilities, and gas and oxygen barrier capabilities. The degradable packaging material has a wide range of uses in the fields of medicine, food, cosmetics, and environmental protection. Especially in the field of pharmaceutical excipients, the hollow capsule made of pullu...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/10C12R1/645
CPCC12P19/10C12N1/145C12R2001/645
Inventor 张慧莉韩蓓佳吕仁杰徐志南
Owner SHIHEZI UNIVERSITY
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