Method for brewing apple wine
A technology of cider and apples, which is applied in the field of drinking wine and cider brewing, and can solve the problems of poor taste of cider and long cider brewing cycle, etc.
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Embodiment 1
[0023] The yeast of the present invention is screened from soil samples under grape vines in a vineyard in Heze, Shandong.
[0024] Weigh 10g of the soil sample and dissolve it in 150mL sterile water, enrich the yeast at 200r / min for 20min at a constant temperature of 28°C, take 1mL of the above shaken culture solution for gradient dilution, and draw 10 -4 、10 -5 and 10 -6 Spread 200 μL of the gradient dilution on the PDA plate, culture it in a constant temperature incubator at 28°C for 24 hours, pick a typical yeast colony and insert it into the YPD liquid medium, and separate it by streaking for 2-3 times, and examine it under the microscope with smear staining. Obtain a pure culture of yeast. Inoculate the yeast with 2% inoculum in an Erlenmeyer flask containing 50mL YPD liquid medium and place it on a 200r / min shaker, and inoculate it on a YPD plate by streaking, and culture the plate and liquid medium at 30°C Smell the flavor after 24 hours (take the YPD plate and liqu...
Embodiment 2
[0027] Embodiment 2 A kind of brewing method of cider, comprises the steps:
[0028] (1) Juicing: Select 5 kg of fresh, highly ripe apples, peel and remove the core and stem to obtain 4.2 kg of pulp, crush and squeeze the juice, and filter and clarify to obtain 3.9 L of clear apple juice.
[0029] (2) Adjust sugar: measure the sugar content of clarified apple juice with an Abbe refractometer, and add sucrose to the apple juice according to the measured sugar content to adjust the sugar content to 20°Brix. , which ensures that the brewed cider has a suitable alcohol content (9°-12°).
[0030] (3) Entering the tank: transfer the 3.9L apple juice with adjusted sugar into the fermentation tank (1) of the brewer, which is the brewer involved in the patent application publication number CN 106281832 A.
[0031] (4) Adding yeast: Saccharomyces cerevisiae (strain preservation number CGMCC No.13443) was shaken and cultured in YPD liquid medium at 28°C for 24 hours, and the yeast liqui...
Embodiment 3
[0038] Embodiment 3 A kind of brewing method of cider, comprises the steps:
[0039] (1) Juicing: Select 5 kg of fresh, highly ripe apples, peel and remove the core and stem to obtain 4.2 kg of pulp, crush and squeeze the juice, and filter and clarify to obtain 3.9 L of clear apple juice.
[0040] (2) Adjust sugar: measure the sugar content of clarified apple juice with an Abbe refractometer, and add sucrose to the apple juice according to the measured sugar content to adjust the sugar content to 25°Brix.
[0041] (3) Entering the tank: transfer the 3.9L apple juice with adjusted sugar into the first fermentation tank 32 of the brewer, which is the brewer involved in the patent application publication number CN 106281832 A.
[0042] (4) Adding yeast: Saccharomyces cerevisiae (strain preservation number CGMCC No.13443) was shaken and cultured in YPD liquid medium at 28°C for 24 hours, and the yeast liquid after the culture was centrifuged in a high-speed centrifuge at 6000r / min...
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