A kind of deer medicine polysaccharide and its preparation method and application

A technology of deer medicine and polysaccharide, which is applied in the field of deer medicine polysaccharide and its preparation, can solve the problems such as no research report on deer medicine polysaccharide, and achieve the effect of promoting the proliferation of pig small intestinal epithelial cells, less pollution and good repeatability

Active Publication Date: 2019-09-13
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition to the above studies, there is no research report on deer medicinal polysaccharides

Method used

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  • A kind of deer medicine polysaccharide and its preparation method and application
  • A kind of deer medicine polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 deer medicine polysaccharide

[0033] A preparation method of the deer medicine polysaccharide of the present invention comprises two steps of extracting the deer medicine polysaccharide and purifying the deer medicine polysaccharide.

[0034] 1. The extraction of deer medicinal polysaccharides comprises the following steps:

[0035] (1) Ultrasonic water extraction: wash the rhizomes and roots of deer medicine, dry them in the shade, crush them, and perform ultrasonic extraction on them with water as a solvent. The effects of material-liquid ratio, ultrasonic temperature, ultrasonic time, and extraction times on the extraction rate were investigated through orthogonal experiments. Finally, the optimal extraction process was obtained: solid-liquid ratio 1:20, ultrasonic temperature 30°C, ultrasonic time 20 min, ultrasonic extraction 3 times, extracted according to this optimal process, combined filtrates, and obtained deer medicine polysac...

Embodiment 2

[0043] The measurement of embodiment 2 deer medicine polysaccharide content

[0044] Utilize ultraviolet spectrophotometry, take glucose as standard substance, adopt phenol-sulfuric acid color development to measure the polysaccharide content of deer medicine, and its specific process is as follows:

[0045] 1. Preparation of phenol solution: Weigh 4 g of freshly distilled phenol, add distilled water to dissolve, make up to 100 mL, mix well, transfer to a brown bottle to obtain 4% phenol solution, and store in the refrigerator for later use.

[0046] 2. Preparation of glucose standard solution: Accurately weigh 200mg of anhydrous glucose standard product dried at 105°C to constant weight, dissolve in distilled water and dilute to 100mL volumetric flask, take 10mL and then dissolve to 100mL, shake well to obtain 0.2mg / mL standard solution.

[0047] 3. Drawing of the standard curve: Precisely measure 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9mL of the standard solution and pl...

Embodiment 3

[0050] Measure the optical rotation of the three deer medicine polysaccharides SJP1-1, SJP2-1, and SJP3-1 with uniform components obtained in Example 1: Weigh 25 mg of the three deer medicine polysaccharides SJP1-1 and SJP2 with uniform components -1. SJP3-1, dissolved in distilled water, fixed to 25mL, using water as blank control, automatic polarimeter to measure optical rotation, calculated, three deer medicine polysaccharides with uniform components SJP1-1, SJP2-1, SJP3 The specific rotations of -1 are -45°, -75°, -35° respectively.

[0051] Three deer drug polysaccharide solutions with uniform components were diluted to measure absorbance, and the specific optical rotation did not change after dilution, which further indicated that SJP1-1, SJP2-1, and SJP3-1 were all deer drug polysaccharides with uniform components.

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Abstract

A deer medicine polysaccharide and its preparation method and application relate to the fields of extraction, separation and purification of natural products, and fill in the blank in the current study of deer medicine polysaccharide components. The method for preparing the deer medicine polysaccharide, first crushing the dried deer medicine rhizome and root, ultrasonically extracting with distilled water, combining the extracts and concentrating them under reduced pressure, then adding absolute ethanol to achieve the volume fraction of ethanol to be 70%, 80% and 90% successively Fractional precipitation, stand still, centrifuge, and freeze-dry to obtain three kinds of crude polysaccharides from deer medicine; deproteinize the above three kinds of crude polysaccharides from deer medicine by Sevag method, separate and purify by DEAE Sepharose FF column chromatography, dialysis, and separate by Sephacryl S‑300 column chromatography After purification, freeze-dry to obtain three deer medicine polysaccharides with uniform components. The invention provides a method for extracting, separating and purifying deer medicine polysaccharides, which is simple in operation, good in repeatability, low in pollution, high in efficiency and good in methodology, and is suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of extraction, separation and purification of natural products, in particular to a deer medicine polysaccharide and its preparation method and application. Background technique [0002] Polysaccharides are macromolecular substances formed by the connection of monosaccharides. They are one of the biological macromolecules necessary for living organisms. play an important role. Due to its special structure, physical and chemical properties and biological activity, polysaccharides are widely used in the field of medicine, such as vaccines, anti-tumor or anti-viral drugs, and the preparation of drug sustained-release agents, medical dialysis membranes, artificial blood, artificial skin, etc. At present, the chemical structure, pharmacological action and mechanism, and structure-activity relationship of polysaccharides have become one of the most active fields in life science research. Polysaccharides extracted...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 赵淑杰路飘飘战杨韩忠明杨利民
Owner JILIN AGRICULTURAL UNIV
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