Preparation method of streptococcus agalactiae type specific antiserum
A Streptococcus agalactiae, specific technology is applied in the field of preparation of Streptococcus agalactiae type-specific antiserum, can solve the problems of lack of methods, low antibody titers, multiple injections of viable bacteria, etc. Low cost, highlighting the effect of large-scale application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0034] Embodiment 1 (preparation of streptococcus agalactiae specific antiserum)
[0035] 1 Antigen preparation
[0036] 1.1 Preparation of inactivated vaccine
[0037] 1.1.1 Preparation of bacteria solution After culturing the freeze-dried strains of the standard strain of Streptococcus agalactiae in nutrient broth for 18 hours, inoculate a blood agar plate containing 7% sterile sheep defibrinated blood for streaking for purification and culture, then pick out the typical A single colony was inoculated in THB broth medium supplemented with 2% newborn bovine serum and 8g / L glucose and subcultured 5 times, and the time of each culture was 6 hours.
[0038] 1.1.2 Preparation of inactivated vaccines The bacteria solution prepared in section 1.1.1 was concentrated by centrifugation at a speed of 4000rpm, centrifugation time was 30min, and the concentration factor was 5 times. Add formaldehyde solution to the concentrated bacteria solution of Streptococcus agalactiae at a ratio o...
Embodiment 2
[0054] Embodiment 2 (preparation of streptococcus agalactiae specific antiserum)
[0055] 1 Antigen preparation
[0056] 1.1 Preparation of inactivated vaccine
[0057] 1.1.1 Preparation of bacteria solution After culturing the freeze-dried strains of Streptococcus agalactiae standard strains in nutrient broth for 12 hours, inoculate a blood agar plate containing 7% sterile sheep defibrinated blood for streaking for purification and culture, and then pick out typical strains. A single colony was inoculated in THB broth medium supplemented with 3% neonatal bovine serum and 4g / L glucose and subcultured 5 times, and the time of each culture was 5 hours.
[0058] 1.1.2 Preparation of inactivated vaccine Preparation 1.1.1 The prepared bacterial solution was concentrated by centrifugation, wherein the centrifugal speed was 6000rpm, the centrifugation time was 20min, and the concentration factor was 7 times. Add formaldehyde solution to the concentrated Streptococcus agalactiae bac...
Embodiment 3
[0074] Embodiment 3 (preparation of streptococcus agalactiae specific antiserum)
[0075] 1 Antigen preparation
[0076] 1.1 Preparation of inactivated vaccine
[0077] 1.1.1 Preparation of bacteria solution After culturing the freeze-dried strains of Streptococcus agalactiae standard strains in nutrient broth for 12 hours, inoculate a blood agar plate containing 7% sterile sheep defibrinated blood for streaking for purification and culture, and then pick out typical strains. The single bacterium colony was inoculated in THB broth medium supplemented with 5% newborn bovine serum and 10g / L glucose and subcultured 5 times, and the time of each culture was 8 hours.
[0078] 1.1.2 Preparation of inactivated vaccine Preparation 1.1.1 The prepared bacterial liquid was concentrated by centrifugation, wherein the centrifugal speed was 8000rpm, the centrifugation time was 15min, and the concentration ratio was 10 times. Add formaldehyde solution to the concentrated bacteria solution ...
PUM
![No PUM](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/noPUMSmall.5c5f49c7.png)
Abstract
Description
Claims
Application Information
![application no application](https://static-eureka-patsnap-com.libproxy1.nus.edu.sg/ssr/23.2.0/_nuxt/application.06fe782c.png)
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap