Method for preparing biological antibacterial organic fertilizer through immunological induction

Abstract

The present invention discloses a method for preparing a biological antibacterial organic fertilizer through immunological induction. According to the present invention, silkworm has super-strong immune protein synthesis ability, wherein the sericin antibacterial peptide is synthesized in a high yield manner through the silk gland on the middle portion of natural fifth instar silkworm, the silk fibroin antibacterial peptide is synthesized through the silk gland on the rear portion, the fibroin gene is regulated by a biological high-activity agent, and synthesis and amplification are performed through a solid-phase or liquid-phase biological technology to prepare the biological antibacterial organic fertilizer; according to the method, the synthesis route of the biological technology is simple and is easy to learn, the product cost is low, the yield can be highly controlled, the disease resistance ability is up to 80-95%, and the obtained product is easily converted and is easily industrialized; and the biological antibacterial organic fertilizer prepared by using the method of the present invention can provide strong antibacterial effects and efficient and broad-spectrum antibacterial advantages for the infections caused by Gram-positive bacteria, Gram-negative bacteria or Bacillus subtilis, Ralstonia solanacearum and pathogenic bacteria, and Fusarium wilt when the biological antibacterial organic fertilizer is stirred in stirrer during agricultural use and is subjected to root soaking before planting.

Description

technical field [0001] The invention relates to a preparation method of a biological antibacterial organic fertilizer, in particular to a method for preparing a biological antibacterial organic fertilizer by immune induction biotechnology. Background technique [0002] The continuous cropping obstacles of some melons, fruits, vegetables and other crops have become the bottleneck restricting agricultural production, resulting in the waste of land resources, manpower, material resources, financial resources and other agricultural inputs, affecting agricultural economic benefits, and also greatly hitting farmers. positivity. Among the continuous cropping soil obstacles of continuous cropping crops, continuous cropping blight mainly caused by fungal pathogens (Fusarium oxysporum) is the most serious obstacle factor. More and more serious; Pepper bacterial wilt caused by R. solanacearum usually causes 20-30% of the death of peppers once it occurs, and when it is severe, it is as...

AI Technical Summary

Problems solved by technology

[0007] However, there is currently no use of immune-inducing biotechnology, using the middle silk gland of the fifth-instar silkworm to synthesize high-volume sericin antimicrobial peptides, synthesizing silk fibroin antimicrobial peptides in the posterior silk gland, and then synthesize and expand through solid-phase or liquid-phase biotechnology. Zeng, research on the preparation of biological antibacterial organic fertilizers for agricultural antibacterial production