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Cytomedicine for preventing intrauterine adhesion and repairing endometrium injury, and preparation method thereof

A cell preparation and endometrial technology, applied in the field of obstetrics and gynecology, can solve the problems of functional recovery of endometrial or short action time, inability to achieve endometrial regeneration and repair, inconvenient cell acquisition, etc., and achieve a lubricating biological barrier. Function, good degradability, growth-promoting and secretion-promoting effects

Inactive Publication Date: 2017-05-31
徐妍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that in the prior art, it is inconvenient to obtain cells, it causes great pain and trauma to patients, and it is difficult to localize when using a single stem cell, the repair is unstable and incomplete, and the traditional intrauterine adhesion and intrauterine Membrane damage repair methods cannot achieve severe endometrial regeneration and repair, endometrial functional recovery or short action time, and poor local curative effect. Provide a mixed stem cell preparation that can effectively prevent intrauterine adhesions and repair severe endometrial damage. and its preparation method

Method used

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  • Cytomedicine for preventing intrauterine adhesion and repairing endometrium injury, and preparation method thereof
  • Cytomedicine for preventing intrauterine adhesion and repairing endometrium injury, and preparation method thereof
  • Cytomedicine for preventing intrauterine adhesion and repairing endometrium injury, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Preparation of cell preparations for preventing intrauterine adhesions and repairing endometrial damage

[0053] The cell preparation for preventing intrauterine adhesions and repairing endometrial damage in this example is a suspension composed of third-generation umbilical cord or placental mesenchymal stem cells, rhSDF-1α and medical sodium hyaluronate gel. And the concentration of each component in the suspension is that the final concentration of umbilical cord or placental mesenchymal stem cells is 5×10 6 individual / mL, the mass concentration of rhSDF-1α was 10 ng / mL, the mass concentration of sodium hyaluronate gel was 10 mg / mL, and the molecular weight of sodium hyaluronate gel was 400,000.

[0054] Wherein, the above-mentioned umbilical cord mesenchymal stem cells are prepared by the following method:

[0055] Take the umbilical cords of full-term cesarean section neonates from volunteers and healthy qualified pregnant women, wash them repeatedly wit...

Embodiment 2

[0058] Example 2 Preparation of cell preparations for preventing intrauterine adhesions and repairing endometrial damage

[0059] The cell preparation for preventing intrauterine adhesions and repairing endometrial damage in this example is a suspension composed of third-generation umbilical cord or placental mesenchymal stem cells, rhSDF-1α and medical sodium hyaluronate gel. And the concentration of each component in the suspension is the final concentration of umbilical cord or placental mesenchymal stem cells 1×10 7 per mL, the mass concentration of rhSDF-1α was 80 ng / mL, the mass concentration of sodium hyaluronate gel was 55 mg / mL, and the molecular weight of sodium hyaluronate gel was 700,000.

[0060] Wherein, the above-mentioned preparation method of umbilical cord mesenchymal stem cells is the same as that in Example 1.

[0061] Wherein, the above-mentioned preparation method of placental mesenchymal stem cells is the same as that in Example 1.

Embodiment 3

[0062] Example 3 Preparation of cell preparations for preventing intrauterine adhesions and repairing endometrial damage

[0063] The cell preparation for preventing intrauterine adhesions and repairing endometrial damage in this example is a suspension composed of third-generation umbilical cord or placental mesenchymal stem cells, rhSDF-1α and medical sodium hyaluronate gel. And the concentration of each component in the suspension is that the final concentration of umbilical cord or placental mesenchymal stem cells is 2×10 8 individual / mL, the mass concentration of rhSDF-1α was 150 ng / mL, the mass concentration of sodium hyaluronate gel was 100 mg / mL, and the molecular weight of sodium hyaluronate gel was 1,000,000.

[0064] Wherein, the above-mentioned preparation method of umbilical cord mesenchymal stem cells is the same as that in Example 1.

[0065] Wherein, the above-mentioned preparation method of placental mesenchymal stem cells is the same as that in Example 1.

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Abstract

The invention relates to a cytomedicine for preventing intrauterine adhesion and repairing endometrium injury, and a preparation method thereof. The cytomedicine consists of umbilical cord or placenta mesenchymal stem cells, recombinant human stromal cell-derived factor-1alpha (rhSDF-1alpha), and sodium hyaluronate gel which is used for bearing the umbilical cord or placenta mesenchymal stem cells and the rhSDF-1alpha and has sustained-release and anti-inflammatory effects. When the cytomedicine is injected into a uterine cavity, the curative effects of promoting stem cells and uterus tissue cells to migrate to the injured part, reducing intimal tissue fibrosis, promoting endometrial regeneration and repair, physically obstructing and preventing intrauterine adhesion and the like are achieved. The problems that the stem cells are singly applied to be difficult in local position as well as the traditional intrauterine adhesion and endometrium injury repair method cannot realize serious endometrium injury repair or is short in action time and poor in local curative effect are solved. The preparation provided by the invention is simple in preparation method, easy to implement, safe and effective, and can doubly improve the concentration of the stem cells in the injured part, prolong the action time of the stem cells and improve local curative effect.

Description

technical field [0001] The invention belongs to the field of obstetrics and gynecology, and relates to a cell preparation for preventing intrauterine adhesion and repairing endometrial damage and a preparation method thereof. Background technique [0002] Intrauterine adhesions (IUA), also known as Asherman syndrome, refers to the formation of adhesion tissue in the uterine cavity due to damage to the basal layer cells of the endometrium caused by trauma, infection, etc., resulting in deformation of the uterine cavity or even complete disappearance of the uterine cavity. , so that patients have abnormal menstruation (amenorrhea or decreased menstrual flow, etc.), infertility, miscarriage, premature delivery and periodic abdominal pain and other abnormal conditions. In recent years, due to frequent uterine cavity operations and popularization of hysteroscopic surgery, the incidence and detection rate of uterine adhesions have gradually increased, and the age of onset tends to...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/54A61L27/20A61L27/22A61L27/52A61L27/50C12N5/0789
CPCA61L27/3834A61L27/20A61L27/227A61L27/3882A61L27/50A61L27/52A61L27/54A61L2300/252A61L2300/412A61L2300/602A61L2400/06C12N5/0665C12N2509/00C08L5/08C08L89/00
Inventor 徐妍陈蕾郭世磊魏翠李红兰王海亚
Owner 徐妍
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