Agent used for preventing and treating apple rot, and method used for preventing and treating apple rot with agent
An apple rot disease prevention and control agent technology, which is applied in the field of apple rot disease prevention and control agents, can solve the problems of great environmental and human hazards, troublesome application and treatment, etc., achieve strong inhibition and killing effects, and prevent apples from being infected with germs
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Embodiment 1
[0012] A preventive agent for apple rot, characterized in that the preventive agent is made from the following steps:
[0013] (1) Mix mulberry leaf powder and lychee juice at 18°Bé at a volume ratio of 3:1. After sterilization, inoculate Phellinus mycelium as a medium, in a dark environment with a humidity of 55% and a temperature of 16°C Carry out expansion culture in medium until the mycelia cover the surface of the medium, collect Phellinus mycelia, mix with citric acid solution with a concentration of 1mol / L in a weight ratio of 1:4, hydrolyze at 60°C for 3 hours, and cool to obtain Hydrolyzate;
[0014] (2) Pass the hydrolyzate through the cation exchange resin to obtain the permeate;
[0015] (3) The permeate is sterilized by high pressure to obtain the preventive agent for apple rot disease.
[0016] When the apple rot control agent is in use, at the beginning of February every year, choose a sunny day, spray the control agent evenly on the surface of the apple tree ...
Embodiment 2
[0018] (1) Mix mulberry leaf powder and lychee juice at 19°Bé at a volume ratio of 3:1. After sterilization, inoculate Phellinus mycelium as a medium, in a dark environment with a humidity of 57% and a temperature of 18°C Carry out expanded culture in medium until the mycelia cover the surface of the culture medium, collect Phellinus mycelium, mix it with a citric acid solution with a concentration of 1.6mol / L at a weight ratio of 1:4.4, hydrolyze it at 67°C for 3.2 hours, cool, get a hydrolyzate;
[0019] (2) Pass the hydrolyzate through the cation exchange resin to obtain the permeate;
[0020] (3) The permeate is sterilized by high pressure to obtain the preventive agent for apple rot disease.
[0021] When the apple rot control agent is in use, at the beginning of February every year, choose a sunny day, spray the control agent evenly on the surface of the apple tree after atomization, the atomization start temperature of the control agent is 83°C, and the atomization end...
Embodiment 3
[0023] (1) Mix mulberry leaf powder and lychee raw juice at 20°Bé in a volume ratio of 3:1. After sterilization, inoculate Phellinus mycelium as a medium, in a dark environment with a humidity of 58% and a temperature of 19°C Carry out expansion culture in medium until the mycelia cover the surface of the culture medium, collect Phellinus mycelium, mix it with a citric acid solution with a concentration of 2mol / L in a weight ratio of 1:5, hydrolyze it at 70°C for 4 hours, and cool to obtain Hydrolyzate;
[0024] (2) Pass the hydrolyzate through the cation exchange resin to obtain the permeate;
[0025] (3) The permeate is sterilized by high pressure to obtain the preventive agent for apple rot disease.
[0026] When the apple rot control agent is in use, at the beginning of February every year, choose a sunny day, spray the control agent evenly on the surface of the apple tree after atomization, the atomization start temperature of the control agent is 85°C, and the atomizati...
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