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Method for detecting bufadienolide components in Liushen pill

A technology for detection of bufadiene and components, which is applied in the detection of traditional Chinese medicine preparations and the field of detection of bufadiene lactones in Liushen Pills. Product quality and other issues, to achieve rapid determination, strong specificity, and good reproducibility

Inactive Publication Date: 2017-05-24
雷允上药业集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the polarity of bufadienolides is very close, the current chromatographic conditions do not meet the method of separating bufadienolides, so the existing technology cannot fully reflect the content of bufadienolides in Liushen Pills Level, can not control the quality of the product very well, there are certain defects

Method used

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  • Method for detecting bufadienolide components in Liushen pill
  • Method for detecting bufadienolide components in Liushen pill
  • Method for detecting bufadienolide components in Liushen pill

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Simultaneous determination of nine bufadienolactones in Liushen Pills by HPLC

[0044] 1 Instruments and reagents

[0045] 1.1 Instrument

[0046] American Waters e2695 high-performance liquid chromatography system, including online degasser, automatic sampler ProminenceSIL-20A, 2998 UV-visible light detector and column thermostat CTO-20A; Hanbang ODS-2 (250mm×4.6mm, 5μm) chromatography Column; Anke TGL-16C high-speed centrifuge (Shanghai Anting Scientific Instrument Factory); electronic analytical balance (FA1204B, Shanghai Precision Science Co., Ltd.); CPA22, 5D electronic balance (Germany Sartorius); Milli-Q Synthesis 108 ultrapure water Instrument (Millipore Corporation); KQ-500DE Ultrasound Machine (Kunshan Ultrasonic Instrument Co., Ltd.).

[0047] 1.2 Reagent

[0048] Acetonitrile (chromatographically pure, American TEDIA Reagent Company), methanol (chromatographically pure, Jiangsu Hanbang Technology Co., Ltd.), redistilled water (made by Milli-Q Synthesis 10...

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Abstract

The invention discloses a method for detecting bufadienolide components in a Liushen pill. The method comprises the following steps: preparing a reference sample solution; preparing a test sample solution; and calculating the content through an HPLC assay determination method and a standard curve method. According to the invention, optimal chromatographic conditions such as mobile phase composition, a chromatographic column, an elution program, detection wavelength and column temperature are screened out through a large number of experiments, and the chromatographic conditions are optimized to obtain the optimal detection conditions. The detection method provided by the invention can realize quick, accurate and reliable determination, has high specificity and favorable reproducibility, can simultaneously detect 9 bufadienolide components in the Liushen pill, and can be used for quality control on the Liushen pill, thereby having important significance for drug quality control.

Description

technical field [0001] The invention relates to a detection method of a traditional Chinese medicine preparation, in particular to a detection method of bufadienolactone components in Liushen Pills, and belongs to the field of component analysis of traditional Chinese medicines. Background technique [0002] Liushen pill is composed of musk, bezoar, toad and other traditional Chinese medicines. It belongs to the national protected traditional Chinese medicine. It has the effect of cooling and detoxifying, reducing inflammation and relieving pain. , carbuncle sore furuncle, milk carbuncle hair back, unknown swelling poison. The medicinal toad venom contained in it is the dry secretion of Bufo bufogargarizans Cantor or B.melanostictus Schneider, a Bufo family animal. Pain relief etc. According to literature reports, the main active ingredients of Bufadienolide are bufadienolactone compounds, which have significant digitalis-like effects, boosting blood pressure, respiratory ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8634
Inventor 杨方钰狄留庆王恒斌严燕青李俊松李全李娟罗年翠
Owner 雷允上药业集团有限公司
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