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Probiotics feed capable of relieving aluminium toxicity of tilapia and application thereof

A technology of probiotics and tilapia, applied in the field of microorganisms, can solve the problems of aquatic bio-aluminum toxicity that have not been reported, and achieve the effect of improving meat quality safety, wide application prospects, and improving intestinal micro-ecological environment

Inactive Publication Date: 2017-05-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, a large number of scholars have studied the beneficial effect of probiotic feed on aquatic animals, but there is still no report on the research on alleviating the toxicity of aluminum in aquatic organisms

Method used

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  • Probiotics feed capable of relieving aluminium toxicity of tilapia and application thereof
  • Probiotics feed capable of relieving aluminium toxicity of tilapia and application thereof
  • Probiotics feed capable of relieving aluminium toxicity of tilapia and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Prepare feed ingredients according to the basic formula shown in Table 1 and the nutritional composition, and the specific steps are as follows:

[0035] (1) Inoculate Lactobacillus plantarum CGMCC No.9664 into MRS medium with an inoculum size of 2-4% by volume, cultivate at 37°C for 18 hours, wash with pH7.2 phosphate buffer for 2-4 times, and use physiological Resuspend in saline to make the concentration of viable bacteria ≥ 10 11 CFU / mL.

[0036] (2) Prepare premixed feed according to the formula in Table 1, specifically by mass percentage: fish meal 10%, secondary meal 25%, cornstarch 20.5%, soybean oil 6.0%, soybean meal 20%, casein 9.6%, gelatin 2.4% , compound vitamin 1%, compound mineral salt 1%, choline chloride 0.5%, VC sodium phosphate 0.2%, calcium dihydrogen phosphate 1.5, microcrystalline cellulose 2.3%;

[0037] Among them, the multivitamin (mg / kg feed) contains: vitamin A, 10; vitamin D, 0,05; vitamin E, 400; vitamin K, 40; vitamin B 1 , 50; Vitamin ...

Embodiment 2

[0042] Example 2 Effects of Lactobacillus plantarum CGMCC No.9664 on growth indicators of aluminum-exposed tilapia

[0043] A total of 192 healthy Nile tilapias were selected, with an initial body weight of 34.01±0.19g (mean±standard deviation). They were randomly divided into four groups, namely the control group, the probiotic group, the metal group and the metal + probiotic group, with 48 animals in each group, and randomly divided into three parallel groups, each with 16 animals. Control group: fed basal diet (that is, basal feed without probiotic ingredients formulated according to Table 1); probiotic group: fed feed containing probiotics (CGMCC No.9664); metal exposure group, fed basal Diets, exposed to metals (2.73mg / L Al 3+ solution) in the water body of the solution; the metal + probiotic (CGMCC No.9664) group was fed with feed containing probiotics and exposed to the water body containing metal aluminum solution. Tilapia were fed twice a day at 3% of the total tila...

Embodiment 3

[0048] Example 3 Effect of Lactobacillus plantarum CGMCC No.9664 on the reduction of aluminum content in aluminum-exposed tilapia

[0049] Carry out animal experiments and grouping with tilapia as described in Example 2, kill the tilapia after the experiment and dissect, take the tissues and organs of each part, put them into a microwave digestion furnace for digestion for 20min, and use the inductive coupling to obtain the digestion solution Aluminum content was determined by plasma mass spectrometry (ICP-MS; NexIon-300X; PerkinElmer). The experimental results are shown in Table 3.

[0050] Table 3 Aluminum content in different parts of tilapia

[0051]

[0052] Note: - means not detected; the unit is μg / g tissue wet weight; groups marked with different letters mean significant difference (P<0.05).

[0053] Comparing the metal + probiotic group with the metal group, it can be found that the probiotics significantly reduced the aluminum content in the gut, liver and splee...

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Abstract

The invention discloses probiotics feed capable of relieving aluminium toxicity of tilapia and application thereof, and belongs to the technical field of microorganism. The probiotics feed is characterized in that a microbial inoculum obtained by performing centrifugal concentration on lactobacillus plantarum CGMCC No. 9664 is added in a feed additive form into a feed premix for aquatic animals for granulating, thus preparing the probiotics granulated feed of a finished feed with the viable count is greater than 108CFU / g. When the fish feed containing lactobacillus plantarum CGMCC No. 9664 is applied to the tilapia, the functions of improving the growth performance of the tilapia, reducing the content of aluminum in tissue and organ and fish of aluminum-exposed tilapia, improving the biochemical criterion of serum, relieving the oxidative stress injury and regulating the intestine microenvironment of aluminum-exposed tilapia are achieved, and the fish feed has a wide application prospect.

Description

technical field [0001] The invention relates to a probiotic feed capable of alleviating the aluminum toxicity of tilapia and an application thereof, belonging to the technical field of microbes. Background technique [0002] Aluminum is the most abundant metallic element in the Earth's crust and is ubiquitous in the environment. In recent years, due to the frequent occurrence of acid rain due to environmental deterioration, more and more aluminum has entered rivers and lakes, causing pollution to water bodies and affecting the health and growth performance of aquatic organisms. Metal aluminum not only has no biological function to living organisms, but also is one of the toxic metals that are extremely harmful to living organisms. Some studies have reported that 0.11-0.22mg / L of aluminum can cause damage to fish. The toxicity of aluminum to aquatic organisms is first manifested in the impact on fish growth performance and survival rate. After fish are exposed to aluminum-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/22A23K10/30A23K20/163A23K20/158A23K10/37A23K20/147A23K20/174A23K10/40A23K20/10A23K20/26A23K50/80
CPCY02A40/818Y02P60/87
Inventor 陈卫田丰伟于雷雷翟齐啸朱家民张程程王刚赵建新刘小鸣张秋香张灏
Owner JIANGNAN UNIV
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