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Seed soaking liquid for promoting seed germination of stevia rebaudiana and preparation method

A seed soaking liquid and stevia technology, applied in botany equipment and methods, preservation of human or animal bodies, chemicals for biological control, etc., can solve the limitation of large-scale planting of stevia and the seed maturity rate of stevia The problems such as low seed germination rate cannot be guaranteed, etc., to achieve good application value, simple preparation method, and good sterilization and disinfection effect

Inactive Publication Date: 2017-05-17
MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, stevia planting mainly includes cutting planting and seed planting. However, the seed maturity rate of stevia is not high, and the germination rate of seeds cannot be guaranteed, which limits the large-scale planting of stevia and is also one of the current shortages of stevia. The reason is, therefore, the research and development of a kind of soaking solution that promotes the emergence of seeds is of great significance for the cultivation of Stevia rebaudiana

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This embodiment relates to a seed soaking solution and a preparation method for promoting the germination of Stevia rebaudiana seeds;

[0022] The seed soaking solution is composed of the following components: 20 parts of Artemisia argyi, 12 parts of Fructus Cnidii, 15 parts of Folium Folium, 10 parts of Galla gall, 12 parts of honeysuckle, 8 parts of licorice, 20 parts of willow twigs, 15 parts of young leaves of poplar, 3 parts of gibberellin.

[0023] The preparation steps of described soaking liquid are as follows:

[0024] (1) Weigh Artemisia argyi, Fructus Cnidii, Folium Folium, Galla Chinensis, Honeysuckle, and Licorice respectively according to the ratio, and grind them to a particle size of 100 mesh, add the crushed powder into the reactor, add the total 20 times the weight of ethanol, heat and reflux extraction at 78°C under ultrasonic conditions for 1.5h, filter and wash the filter residue;

[0025] (2) Repeat the extraction operation of step (1) twice, com...

Embodiment 2

[0030] This embodiment relates to a seed soaking solution and a preparation method for promoting the germination of Stevia rebaudiana seeds;

[0031] The seed soaking solution consists of the following components: 10 parts of Artemisia argyi, 10 parts of Fructus Cnidii, 10 parts of Folium Folium, 5 parts of Galla gall, 10 parts of honeysuckle, 5 parts of licorice, 10 parts of willow twigs, 10 parts of young leaves of poplar, 1 part gibberellin.

[0032] The preparation steps of described soaking liquid are as follows:

[0033] (1) Weigh Artemisia argyi, Fructus Cnidii, Folium Folium, Galla Chinensis, Honeysuckle, and Licorice respectively according to the ratio, and grind them to a particle size of 100 mesh, add the crushed powder into the reactor, add the total 15-20 times the weight of ethanol, heat and reflux at 80°C for 1 hour under ultrasonic conditions, filter and wash the filter residue;

[0034] (2) Repeat the extraction operation of step (1) twice, combine the filtr...

Embodiment 3

[0039] This embodiment relates to a seed soaking solution and a preparation method for promoting the germination of Stevia rebaudiana seeds;

[0040] The seed soaking solution is composed of the following components: 30 parts of Artemisia argyi, 15 parts of Fructus Cnidii, 20 parts of Folium Folium, 15 parts of Galla gall, 15 parts of honeysuckle, 10 parts of licorice, 30 parts of willow twigs, 20 parts of young leaves of poplar, Gibberellin 5 parts.

[0041] The preparation steps of described soaking liquid are as follows:

[0042] (1) Weigh Artemisia argyi, Fructus Cnidii, Folium Folium, Galla Chinensis, Honeysuckle, and Licorice according to the ratio, and grind them to a particle size of 150 mesh, add the crushed powder into the reactor, add the total 20 times the weight of ethanol, heat and reflux at 75°C for 2 hours under ultrasonic conditions, filter and wash the filter residue;

[0043] (2) Repeat the extraction operation of step (1) twice, combine the filtrate and w...

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Abstract

The invention relates to the technical field of agricultural seed breeding, and particularly discloses a seed soaking liquid for promoting seed germination of stevia rebaudiana and a preparation method. The preparation method comprises the following steps: respectively weighing folium artemisiae argyi, fructus cnidii, folium isatidis, gallnut, honeysuckle and liquorice, crushing, adding into a reactor, adding a solvent, namely, ethanol, performing heating reflux extraction for 1-2 hours under ultrasonic conditions, filtering, and washing filter residues; repeating the extraction operator twice, and combining filtrate and washing liquor so as to obtain a mixed extraction liquid; respectively weighing tender willow twigs and tender aspen leaves, smashing in a container, adding water to soak for 1-2 hours at the room temperature, and filtering so as to obtain a soaking liquid; extracting a mixed liquid and a soaking liquid, combining the liquids, concentrating, adding gibberellins into the mixed concentrated liquid, and dissolving sufficiently, so as to obtain the seed soaking liquid. By adopting the seed soaking liquid disclosed by the invention, seed germination can be effectively promoted, a relatively good sterilization and disinfection function can be achieved; the preparation method is simple, convenient to operate, applicable to popularization and application, and relatively good in application value.

Description

technical field [0001] The invention relates to the technical field of agricultural seed breeding, in particular to a seed soaking solution for promoting the germination of Stevia rebaudiana seeds and a preparation method thereof. Background technique [0002] Stevia is a perennial herbaceous plant of Compositae, native to subtropical South America. The roots, stems and leaves of Stevia rebaudiana contain steviol glycosides. Stevioside has the characteristics of high sweetness (200-300 times that of sucrose), low calorie (only 1 / 300 of sucrose), and can increase blood sugar, lower blood pressure, and promote metabolism. Heart disease, dental caries, etc. have a certain auxiliary effect, so it has increasingly attracted people's attention and attention, and has become the "third sugar source" after sucrose and synthetic sugar. [0003] In recent years, with the increase in demand for stevia, the planting area is also expanding. It is planted in Hainan in the south, Heilongj...

Claims

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Application Information

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IPC IPC(8): A01N25/02A01N65/20A01N63/02A01N43/12A01P21/00A01P1/00A01P3/00
CPCA01N25/02A01N43/12A01N63/10A01N65/00A01N65/20A01N2300/00
Inventor 贾德军
Owner MINGGUANG DAQUAN STEVIA PROFESSIONAL COOP
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