Staining agent for staining horse radish peroxidase and preparation method of staining agent, staining composition, application and marker enzyme staining kit

Abstract

The invention relates to the technical field of biology and provides a staining agent for staining horse radish peroxidase and a preparation method of the staining agent, a staining composition, an application and a marker enzyme staining kit. The staining composition comprises the following reagents: diaminobenzidine, nickel ammonium sulfate, glucose, ammonium chloride, glucose oxidase, acetic acid and sodium acetate. The staining composition can be applied to preparation of the marker enzyme staining kit. The kit comprises the staining composition. The staining agent is prepared from the staining composition by the processes of preparing a diaminobenzidine water solution from diaminobenzidine as a first mixture; preparing a nickel ammonium sulfate water solution from nickel ammonium sulfate as a second mixture; mixing the glucose, the ammonium chloride and the glucose oxidase to form a third mixture; preparing a sodium acetate water solution from the acetic acid and the sodium acetate as a fourth mixture; and mixing the first mixture and the second mixture, then mixing with the third mixture and finally mixing with the fourth mixture. The preparation method is simple in procedure.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a sensitive staining agent for horseradish peroxidase staining, a preparation method, a staining combination, an application and a labeling enzyme staining kit. Background technique [0002] Horseradish peroxidase (HRP) is the most commonly used qualitative and quantitative labeling enzyme in biological, medical and pharmaceutical experiments and detection. It can be used to label proteins, nucleic acids, viruses, tumor factors, drugs, poisons, cells Factors, antibodies, antigens, receptors, and specific components in blood, cerebrospinal fluid, and tissues, etc. HRP itself has no color, and the nature and content of the detected substance can be qualitatively or quantitatively developed only by dyeing method. [0003] The most commonly used HRP staining method is diaminobenzidine staining. But this method has the following disadvantages: (1) the method is not sensitive, the dyeing...

AI Technical Summary

Problems solved by technology

But this method has the following disadvantages: (1) the method is not sensitive, the dyeing of markers is not deep, it is difficult to dye a small amount of detection substances, and it is easy to produce false negative results, and it is easy to draw false conclusions that there is no object to be tested; (2) The specificity of this method is poor, and the part without markers may also be dyed yellow, and the non-specific substances are dyed together, which is easy to get a false positive result, resulting in a wrong result that is mistaken for the detected object; (3) the method The steps are complicated, the operation is troublesome, time-consuming and labor-intensive

In short, the resolution of HRP staining by this method is not high, and it is easy to cause misjudgment

Images