Fast detection method of aminohydantoin hydrochloride

A nitrofuracil and detection method technology, applied in the detection field, can solve the problems of high degree of instrumentation, high price, complicated processing process, etc., and achieve the effects of high sensitivity, large detection sample volume, and short detection time.

Inactive Publication Date: 2017-05-10
WUXI X RES PROD DESIGN & RES
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  • Abstract
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Problems solved by technology

At present, the most common methods used to detect nitrofuran metabolites are high performance liquid chromatography (HPLC), liquid chromatography-mass chromatography (LC-MS / MS), etc. These methods have high sensitivity, accurate results, good repeatability, and false positives. There are few positives, but the sample pretreatment process is complicated, the degree of instrumentation is high, and the price is expensive

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The rapid detection method of nitrofurazone metabolite of the present invention, comprises the steps: (a) make fluorescent sensitivity standard curve: prepare the standard solution of nitrofurazone metabolite of a series of different concentrations, and it is moved in the colorimetric tube of 5mL respectively, to Add 1.0mL concentration of 7.25× to each colorimetric tube mol / L CdTe solution, constant volume with phosphate buffer solution of pH=7.4, shake well, place at room temperature for 5min, detect the fluorescence intensity F of each of the above systems with a molecular fluorescence photometer; at the same time, take 1.0mL concentration of 7.25 mol / L CdTe solution was added to a 5mL colorimetric tube, and the phosphate buffer solution with pH = 7.4 was used to make up the volume, after shaking well, it was placed at room temperature for 15 minutes, and the fluorescence intensity F0 of the system was detected by a molecular fluorescence photometer; Concentration ...

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Abstract

The invention discloses a fast detection method of aminohydantoin hydrochloride. The fast detection method comprises the following steps: (a) manufacturing a fluorescence sensitization standard curve: preparing a series of aminohydantoin hydrochloride standard solutions with different concentrations, and respectively transferring each aminohydantoin hydrochloride standard solution to a colorimetric tube of 5mL, adding 1.0mL of CdTe solution with concentration of 7.25*10<-4> mol / L in each colorimetric tube, metering volume by use of a phosphate buffer solution with pH of 7.4, uniformly shaking, placing at room temperature for 5-10min, and detecting the fluorescence intensity F of each system by use of a molecular fluorophotometer; and meanwhile, adding 1.0mL of CdTe solution with concentration of 7.25*10<-4> into the colorimetric tube of 5mL. The fast detection disclosed by the invention has the characteristics of being high in sensitivity, simple in sample preprocessing, transient in detection time, and large in detection sample amount, and suitable for on-site supervision and the screening of mass samples.

Description

technical field [0001] The invention relates to a detection method, in particular to a rapid detection method of nitrofurazone metabolites. Background technique [0002] Nitrofurans are often used as broad-spectrum antibiotics to prevent and treat gastrointestinal diseases in pigs, cattle, poultry and bees caused by Salmonella and Escherichia. However, in the course of long-term experimental research, it was found that both nitrofuran drugs and metabolites can cause cancer and gene mutations in experimental animals, and such drugs are prohibited from being used in treatment and feed. Since the prototype drug of nitrofuran is rapidly metabolized in the body, it cannot be detected, but its metabolites are quite stable due to the combination with proteins, so when analyzing the residues of such drugs, it is often necessary to analyze the metabolized products, and the management department uses The detection of metabolites is a means to achieve the purpose of detection of nitro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 吴敏芳徐静赵春城胡勇蒋韦艳刘金杰朱倩倩
Owner WUXI X RES PROD DESIGN & RES
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