Preparation method of sea urchin peptide oral liquid

A technology for oral liquid and sea urchin, which is applied in the field of preparation of sea urchin-like peptide oral liquid, can solve the problem of high production cost in fishing season, and achieve the effects of low price, full umami and honest taste.

Inactive Publication Date: 2017-05-10
山东明鑫集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of the existing active peptides prepared from sea urchin yellow as raw materials and the products are easily affected by fishing seasons and high production costs, the present invention provides a sea urchinoid with reasonable process, easy operation, unlimited source of raw materials, and low production cost. A prepar...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A sea urchin-like peptide oral liquid and a preparation method thereof, through the following process steps:

[0048] (1) Extraction of sea urchin yellow cell RNA

[0049] (1.1), homogenate treatment

[0050] Weigh 150mg of echinacea tissue and grind it in liquid nitrogen, add 1.5ml TRIzol, and use a homogenizer for homogenization;

[0051] (1.2), stand still

[0052] The homogenized mixture obtained in step (1.1) was allowed to stand at room temperature at 20°C for 4 minutes to completely separate the nucleic acid-protein complex;

[0053] (1.3), centrifuge to remove impurities

[0054] Centrifuge the mixture obtained in step (1.2) at 10,000 g for 9 min at 5°C, and collect the supernatant;

[0055] (1.4), extraction

[0056] Add 16% chloroform by volume to the supernatant obtained in step (1.3), shake vigorously for 18 seconds, and let stand at room temperature for 3 minutes;

[0057] (1.5), centrifugal

[0058] Centrifuge the mixture obtained in step (1.4) at 1...

Embodiment 2

[0083] A sea urchin-like peptide oral liquid and a preparation method thereof, through the following process steps:

[0084] (1) Extraction of sea urchin yellow cell RNA

[0085] (1.1), homogenate treatment

[0086] Weigh 200mg of echinacea tissue and grind it in liquid nitrogen, add 2ml TRIzol, and use a homogenizer for homogenization;

[0087] (1.2), stand still

[0088] The homogenized mixture obtained in step (1.1) was allowed to stand at room temperature at 30°C for 3 minutes to completely separate the nucleic acid-protein complex;

[0089] (1.3), centrifuge to remove impurities

[0090] Centrifuge the mixture obtained in step (1.2) at 10,000 g for 8 min at 8°C, and collect the supernatant;

[0091] (1.4), extraction

[0092] Add 20% volume of chloroform to the supernatant obtained in step (1.3), shake vigorously for 20 seconds, and let stand at room temperature for 3 minutes;

[0093] (1.5), centrifugal

[0094] Centrifuge the mixture obtained in step (1.4) at 10,...

Embodiment 3

[0119] A sea urchin-like peptide oral liquid and a preparation method thereof, through the following process steps:

[0120] (1) Extraction of sea urchin yellow cell RNA

[0121] (1.1), homogenate treatment

[0122] Weigh 100mg of echinacea tissue and grind it in liquid nitrogen, add 1ml TRIzol, and use a homogenizer for homogenization;

[0123] (1.2), stand still

[0124] The homogenized mixture obtained in step (1.1) was allowed to stand at room temperature at 15°C for 5 minutes to completely separate the nucleic acid-protein complex;

[0125] (1.3), centrifuge to remove impurities

[0126] Centrifuge the rested mixture obtained in step (1.2) at 10000g for 10min at 2°C, and collect the supernatant;

[0127] (1.4), extraction

[0128] Add 10% chloroform by volume to the supernatant obtained in step (1.3), shake vigorously for 15 seconds, and let stand at room temperature for 2 minutes;

[0129] (1.5), centrifugal

[0130] Centrifuge the mixture obtained in step (1.4) a...

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Abstract

The invention discloses a preparation method of sea urchin peptide oral liquid and aims at solving the problems that sea-urchin gonad can be easily influenced by catching season and the like when being taken as a raw material in the prior art and production cost is high. The preparation method comprises the following steps: selecting sea-urchin gonad tissues, and obtaining RNA of sea-urchin gonad cells by adopting a chloroform extraction method; then adopting an RT-PCR method to obtain cDNA of the sea-urchin gonad cells, then carrying out PCR by utilizing a random primer to obtain all kinds of random gene fragments, then cloning the obtained gene fragments into a prokaryotic expression vector, carrying out induced expression to obtain random protein encoded by a random gene, carrying out protease digestion, and carrying out ultrafiltration to obtain sea urchin peptide solution; and blending the sea urchin peptide solution with wolfberry extract and mint extract to obtain the sea urchin peptide oral liquid. The obtained oral liquid is rich in lycium barbarum polysaccharide, organic acid, glycosides and flavones, taste is mellow, sweetness and acidity are moderate, antioxidant activity is good, and nutritional value is high; and technology is reasonable, operation is easy, production cost is low, and large-scale production can be easily realized.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a preparation method of sea urchin-like peptide oral liquid. Background technique [0002] In recent years, research on reactive oxygen species (ROS) has been increasing. Many studies have confirmed that active oxygen free radicals are related to the generation of cancer, liver disease, Alzheimer's disease, arthritis, diabetes and other diseases. All compounds that can interfere with chain initiation and chain growth in free radical chain reactions and scavenge ROS are collectively called antioxidants. There are two types of antioxidants: endogenous and exogenous. The former includes antioxidant enzymes, uric acid, ubiquinone, glutathione, etc. produced during the metabolism of organisms, and the latter includes some synthetic or natural antioxidants, such as butyl hydroxyanisole, plant polyphenols, etc. Since the safety of chemically synthesized phenolic antioxidants h...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12N15/66C12N15/70A23L17/00A23J3/34A23L33/105
Inventor 孙治政刘建文姜军强王菁
Owner 山东明鑫集团有限公司
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