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Method for irradiation-mutating japonica rice in Northeast China through heavy-ion beam C

A heavy ion beam and irradiation technology, which is applied in botany equipment and methods, rice cultivation, plant genetic improvement, etc., can solve the problems of low mutation rate and favorable mutation rate in the method of heavy ion beam irradiation, and shorten the stable speed , increase the mutation rate, and have more beneficial types

Inactive Publication Date: 2017-05-10
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problem of low mutation rate and favorable mutation rate in the existing heavy ion beam irradiation method, and provides a method for mutagenizing northeast japonica rice by heavy ion beam C irradiation

Method used

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specific Embodiment approach 1

[0016] Specific embodiment one: the method for the heavy ion beam C irradiation mutagenesis Northeast japonica rice of the present embodiment comprises the following steps:

[0017] 1. Northeast japonica rice varieties with a growth period of more than 140 days, excellent comprehensive traits, and more than 5 years of promotion but with horizontal resistance to rice blast are selected as irradiation materials;

[0018] 2. The equipment used is a heavy ion accelerator. The heavy ion beam C irradiates the embryos of Northeast japonica rice. The number of irradiated grains is 200-250. Sample time is 15 minutes;

[0019] 3. Irradiate contemporary M according to step 2 0 Seeds, after normal sowing, the surviving seedlings are transplanted in a single copy, and when they mature, all plants M 1 are semi-sterile, the M 1 All the solid grains above are mixed, harvested, threshed and preserved;

[0020] 4. Next spring, M 1 All single plants are planted as M 2 Generation, while pla...

specific Embodiment approach 2

[0023] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the heavy ion accelerator mentioned in step 2 is from the Lanzhou Heavy Ion Accelerator of the Chinese Academy of Sciences. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0024] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: in step three, the row spacing of a single transplant of surviving rice seedlings is 30 centimeters × 20 centimeters. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for irradiation-mutating japonica rice in Northeast China through aheavy-ion beam C, in particular to a breeding method of japonica rice. The method for irradiation-mutating the japonica rice in Northeast China through the heavy-ion beam C provided by the invention aims to solve the problem of low mutation rate and beneficial mutation rate of an existing heavy-ion beam irradiation method. The method comprises the steps of (1) selecting japonica rice varieties in Northeast China as irradiation materials; (2) adopting a heavy-ion accelerator as an instrument, and using the heavy-ion beam C for irradiating germ surfaces of the japonica rice in Northeast China; (3) irradiating current M0 seeds, then normally sowing, transplanting survived rice seedling individual plants, mixed harvesting, threshing and storing all seed grains on M1; (4) in the spring of next year, planting M1 individual plants to obtain M2 generation, and appearing mutated plants; (5) in the winter of the year, adding-generation planting variant plants harvested from the M2-generation individual plants to obtain M3 generation in Sanya, Hainan; (6) in the spring of next year, planting the individual plants harvested from M3 to obtain M4 generation, monitoring yield, testing seeds, and selecting materials with the roughness factor being not less than 77 percent, the head rice rate being not less than 62 percent, the amylose being ranged from 15 to 20 percent, and the chalkiness percentage being below 20 percent. The method provided by the invention is used for breeding the japonica rice in Northeast China.

Description

technical field [0001] The invention relates to a breeding method of northeast japonica rice. Background technique [0002] Practice in the past few decades has proved that mutagenesis technology is an effective way and method for crop germplasm innovation and variety improvement. As a new radiation source, heavy ion beam has advantages that conventional radiation sources do not have, and it is a new technology for mutation breeding. It not only has the characteristics of large energy transfer line density, high relative biological effect, small repair effect after damage, wide mutation spectrum, fast stabilization of the traits of offspring mutant materials, and short breeding cycle, but also can utilize its local interaction with organisms. Sex, controllability and selectivity for directional breeding. [0003] Longfu 2, a new spring wheat variety with the characteristics of high yield, stable yield, wide adaptability, and excellent quality, was previously bred by heavy ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01G16/00A01G22/22
CPCA01H1/06A01G22/22
Inventor 杨福李景鹏
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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