Method for irradiation-mutating japonica rice in Northeast China through heavy-ion beam C
A heavy ion beam and irradiation technology, which is applied in botany equipment and methods, rice cultivation, plant genetic improvement, etc., can solve the problems of low mutation rate and favorable mutation rate in the method of heavy ion beam irradiation, and shorten the stable speed , increase the mutation rate, and have more beneficial types
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specific Embodiment approach 1
[0016] Specific embodiment one: the method for the heavy ion beam C irradiation mutagenesis Northeast japonica rice of the present embodiment comprises the following steps:
[0017] 1. Northeast japonica rice varieties with a growth period of more than 140 days, excellent comprehensive traits, and more than 5 years of promotion but with horizontal resistance to rice blast are selected as irradiation materials;
[0018] 2. The equipment used is a heavy ion accelerator. The heavy ion beam C irradiates the embryos of Northeast japonica rice. The number of irradiated grains is 200-250. Sample time is 15 minutes;
[0019] 3. Irradiate contemporary M according to step 2 0 Seeds, after normal sowing, the surviving seedlings are transplanted in a single copy, and when they mature, all plants M 1 are semi-sterile, the M 1 All the solid grains above are mixed, harvested, threshed and preserved;
[0020] 4. Next spring, M 1 All single plants are planted as M 2 Generation, while pla...
specific Embodiment approach 2
[0023] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the heavy ion accelerator mentioned in step 2 is from the Lanzhou Heavy Ion Accelerator of the Chinese Academy of Sciences. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0024] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that: in step three, the row spacing of a single transplant of surviving rice seedlings is 30 centimeters × 20 centimeters. Others are the same as in the first or second embodiment.
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