Nano multidirectional chromatography nucleic acid extraction medium and preparation method thereof

An extraction medium and nano-technology, which is applied in DNA preparation, chemical instruments and methods, and other chemical processes, can solve the problems of complicated steps, storage of liquid nucleic acid, harsh conditions for transportation, and unreliable test results, etc., so as to facilitate transportation , prevent degradation, good integrity effect

Active Publication Date: 2017-05-03
SUZHOU HAIMIAO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The steps are relatively complicated. In addition, the conditions for storage and transportation of liquid nucleic acid are relatively strict, and liquid nucleic acid will be degraded during storage, resulting in unreliable test results in the later stage.

Method used

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  • Nano multidirectional chromatography nucleic acid extraction medium and preparation method thereof
  • Nano multidirectional chromatography nucleic acid extraction medium and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The first step: provide a base material for adsorption of one square centimeter, and dry the base material. The drying method is: dry in a vacuum at -5°C-5°C, and then place it at room temperature and relative humidity ≤ 30% to reach equilibrium;

[0021] The second step: under the condition of room temperature relative humidity ≥ 80%, drop the first buffer solution on the substrate until the substrate is saturated with water. Wherein, guanidine hydrochloride accounts for 0.7% of the buffer solution, SDS accounts for 0.7% of the buffer solution, and EDTA accounts for 0.06% of the buffer solution.

[0022] Specifically, 100ul (one drop) of buffer solution each time, weighed with an analytical balance, and confirmed whether the water absorption of the substrate is saturated. When the mass difference between two adjacent times is less than 0.001g, the surface substrate has saturated water absorption. As shown in the following table:

[0023]

[0024]

[0025] It has...

Embodiment 2

[0041]The first step: provide a base material for adsorption of one square centimeter, and dry the base material. The drying method is: dry in a vacuum at 5°C, and then place it at room temperature and relative humidity ≤ 30% to achieve equilibrium;

[0042] The second step: under the condition of room temperature relative humidity ≥ 80%, drop the first buffer solution on the substrate until the substrate is saturated with water. Wherein, guanidine hydrochloride accounts for 10.0% of the buffer solution, SDS accounts for 10.2% of the buffer solution, and EDTA accounts for 6.0% of the buffer solution.

[0043] Step 3: Dry the substrate to which the first buffer solution has been dropped for 30 minutes at a room temperature with a relative humidity of ≤20%.

[0044] Step 4: Add a protective agent with 100 mg / ml hydroxyethyl starch dropwise to the substrate to achieve a liquid volume of 30-50 ul per square centimeter of the substrate, and the protective agent is completely absorb...

Embodiment 3

[0051] The first step: provide a base material for adsorption of one square centimeter, and dry the base material. The drying method is: dry in a vacuum at 0°C, and then place it at room temperature and relative humidity ≤ 30% to achieve equilibrium;

[0052] The second step: under the condition of room temperature relative humidity ≥ 80%, drop the first buffer solution on the substrate until the substrate is saturated with water. Wherein, guanidine hydrochloride accounts for 5.0% of the buffer solution, SDS accounts for 5.0% of the buffer solution, and EDTA accounts for 3.0% of the buffer solution.

[0053] Step 3: Dry the substrate to which the first buffer solution has been dropped for 30 minutes at a room temperature with a relative humidity of ≤20%.

[0054] Step 4: Add a protective agent with 100 mg / ml hydroxyethyl starch dropwise to the substrate to achieve a liquid volume of 30-50 ul per square centimeter of the substrate, and the protective agent is completely absorbe...

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Abstract

The invention discloses a nano multidirectional chromatography nucleic acid extraction medium, which has a substrate of a macromolecular carbohydrate and an extract penetrating the substrate. The extract comprises a mixture of guanidine hydrochloride, SDS and EDTA located at a first layer, a protective agent located at a second layer, a mixture of guanidine hydrochloride, SDS and EDTA located at a third layer, and a protective agent located at a fourth layer. And the mixture of guanidine hydrochloride, SDS and EDTA located at the first layer and the mixture of guanidine hydrochloride, SDS and EDTA located at the third layer are different in content. The substrate can well preserve the nucleic acid integrity and prevent nucleic acid degradation.

Description

technical field [0001] The invention relates to a nucleic acid extraction material, in particular to a nanometer multidirectional chromatography nucleic acid extraction medium and a preparation method thereof. Background technique [0002] At present, the principle of nucleic acid extraction is generally to ensure the integrity of the primary structure of nucleic acid and exclude contamination from other molecules. Issues that should be paid attention to in nucleic acid extraction: Simplify the steps, shorten the extraction time, reduce the degradation of nucleic acids by chemical factors, and reduce the degradation of nucleic acids by physical factors: mechanical shear force and high temperature, to prevent the biodegradation of nucleic acids, nucleic acid extraction can be roughly divided into 3 major categories Parts: crushing, extraction, purification. [0003] Before crushing and extraction, the existing technology is to use different containers to collect / collect vari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/32C12N15/10
CPCB01J20/265B01J20/3289C12N15/101
Inventor 谭淼
Owner SUZHOU HAIMIAO BIOTECH CO LTD
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