Novel biological seed coating and application thereof
A biological species, bacillus technology, applied in the direction of biocide, application, biocide, etc., can solve the problems of poor product quality, single variety, weak biological seed coating market, etc., to reduce application amount, develop root system, and improve germination. The effect of efficiency and quality
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Embodiment 1
[0024] The preparation method of embodiment 1 microbial mixed fermented liquid
[0025] 1. Activate Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus respectively; inoculate the activated Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus into the seeds in sequence according to the ratio of 2-5% Culture medium (LB medium) for mixed culture; under the conditions of 35°C and 120rpm / min, culture on a shaking table for 36-48 hours to obtain the first-grade seed liquid;
[0026] 2. According to the inoculation amount of 10-15%, transfer the primary seed solution to the secondary seed medium in the seed tank (each component and its mass ratio are: starch 1.5-3%, bran powder 1-2 %, yeast extract 0.05-0.3%, peptone 0.5-1%, phosphate rock powder 1-2%, MgSO 4 ·7H 2 O 0.01~0.05%, CaCl 2 0.1~0.4%, (NH4) 2 SO 4 0.01~0.04%, the balance is water, pH 7.0~7.2), cultivated at 28~37℃, with a rotation speed of 100rpm / min, an air flow of 1000L / h, and a tank pressure of ...
Embodiment 2
[0028] The preparation method of embodiment 2 biological seed coating agent
[0029] A biological seed coating agent, the components and contents of which are: the mixed fermentation liquid of Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus (the number of viable bacteria is about 10 10 CFU / mL, the ratio of the number of viable bacteria of the three bacteria is about 4:3:2), the contents of phytase, sodium alginate, starch, calcium carbonate, Tween 80 and pigment are respectively phytase 2000U / mL, Sodium alginate 9% (w / v), sodium carboxymethylcellulose 3% (w / v), starch 4% (w / v), Tween 80 3% (v / v), calcium carbonate 1%, Pigment 0.3% (w / v);
[0030] Name it No. 1 seed coating agent.
[0031] Weigh each raw material according to the raw materials in the above-mentioned biological seed coating agent, first heat the water to 50°C, stir sodium alginate and starch into the water, dissolve the film-forming solvent completely, then add calcium carbonate, Tween Mix 80 an...
Embodiment 3
[0033]A biological seed coating agent, its components and contents are: a biological seed coating agent, its components and contents are: the mixed fermentation liquid of Paenibacillus polymyxa, Bacillus cereus and Bacillus pumilus (live The number of bacteria is about 10 10 CFU / mL, the ratio of the number of live bacteria of the three kinds of bacteria is about 3:2:2), the contents of phytase, sodium alginate, starch, calcium carbonate, Tween 80 and pigment are respectively phytase 3000U / mL, Sodium alginate 6% (w / v), sodium carboxymethylcellulose 5% (w / v), starch 4.5% (w / v), Tween 80 4% (v / v), calcium carbonate 1.5%, Pigment 0.3% (w / v); name it No. 2 seed coating agent.
[0034] The preparation method is the same as in Example 2.
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