Sterile micro grafting method of koelreuteria bipinnata var. intergrifoliola 'Jinyan'
A technology of golden flame colorful luan and micro-grafting, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as affecting subculture, difficulty in mastering operation techniques, and high survival rate, so as to improve survival rate and The effect of the germination effect
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Embodiment 1
[0013] The rootstock culture is to adopt excellent single-plant tissue culture of Huangshan eucalyptus. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75% Soak in alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. 3. The shoots of 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L+sucrose 30g / L; For scion culture, inoculate the buds of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is improved 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L + 30g / L sucrose, the cultivation time is 15-45 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stems with la...
Embodiment 2
[0015] The rootstock culture is to adopt excellent single-plant tissue culture of Huangshan eucalyptus. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75% Soak in alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. 3. The shoots of 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L+sucrose 30g / L; For scion culture, inoculate the buds of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is improved 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L + 30g / L sucrose, the cultivation time is 15-45 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stems with la...
Embodiment 3
[0017] The rootstock culture is to adopt excellent single-plant tissue culture of Huangshan eucalyptus. Before the tissue culture, the dust on the surface of the material is cleaned, and then cut into 1-1.5cm stem segments with lateral buds, and the surface is sterilized. When sterilizing, first use 75% Soak in alcohol for about 10 seconds, rinse twice with sterile water, then sterilize with 0.1% HgCl for 5-6 minutes, and then rinse with sterile water for 3 times. 3. The shoots of 4 leaves are used as rootstock grafting, and the medium is 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L+sucrose 30g / L; For scion culture, inoculate the buds of Jinyancailuan seedlings in mid-April to inoculate the tissue culture buds, and the medium is improved 1 / 2MS+6-BA 0.0 2mg / L+NAA 0.05mg / L++IAA 0.05mg / L+IBA 0.02mg / L + 30g / L sucrose, the cultivation time is 15-45 days, the dust on the surface of the material is cleaned before tissue culture, and then cut into 1-1.5cm stems with la...
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