Method for callus efficient induction seedling development of stemona sessilifolia

Abstract

The invention discloses a method for callus efficient induction seedling development of stemona sessilifolia. The method comprises the following steps: taking a stemona sessilifolia tender shoot as an explant, and putting the explant into an MS primary induction culture medium to perform induction so as to obtain a sterile test-tube plantlet; putting the test-tube plantlet into an MS callus induction culture medium to perform callus culture; putting an obtained callus into an MS differential culture medium to obtain a large amount of cluster buds; putting the cluster buds into an MS sound seedling culture medium to perform sound seedling culture; and putting obtained robust plants into an MS rooting culture medium to perform rooting culture. A test shows that by adopting the propagation method provided by the invention, the multiplication coefficient of the obtained cluster buds is 40-80 times, the rooting rate of the obtained tissue culture seedlings is 95% or above, and the survival rate of the seedlings transplanted to a seedbed is 90% or above. Therefore, the method disclosed by the invention can make effective use of existing resources to quickly propagate the stemona sessilifolia, solves the problem of industrialized rooting and seedling raising of the stemona sessilifolia, and can fully guarantee a raw material source for medical application development of the stemona sessilifolia.

Description

technical field [0001] The invention belongs to the field of tissue culture of C. erectus, in particular to a method for efficiently inducing seedlings of C. erectus callus. Background technique [0002] Stemona sessilifolia (Miq.) Miq. is a plant of the genus Stemona sessilifolia, produced in Zhejiang, Jiangsu, Anhui, Jiangxi, Shandong, Henan and other provinces. The tuber root of Baibu erecta is one of the three legal sources of the traditional Chinese medicine Baibu recorded in the "Chinese Pharmacopoeia" (2015 edition). Cough; externally used for head lice, body lice, pinworms, pruritus vulvae. Studies have shown that the main active ingredients of Libucia erecta are a unique class of Libucia alkaloids with a pyrrole or pyridazazulene mother nucleus structure, such as the roots containing Libucaine, Protobubene, Libucaine Bubudine, isoprotine, erectine, hodolinine, phyllotine, etc. Modern pharmacological studies have shown that alkaloids of basil have the functions of...

AI Technical Summary

Problems solved by technology

At the same time, due to the limited distribution range of the plant, it is almost difficult to find commercial medicinal materials in the market, so it is imperative to plant and raise the plant manually.

At present, although there are about the tissue culture of upright plant stems (patent application number 201410540445.9 published on December 24, 2014) and the same family and the same genus plant plant stems (Li Kelie, creeping stem plant tissue culture rapid propagation technology, Guangxi Agricultural Science, 2005, 36:505-506) and related reports on the tissue culture of Phyllostachys chinensis (Yang Zhende, Research on tissue culture and rapid propagation technology of Phyllostachys chinensis, Modern Agricultural Science and Technology, 2016, 2: 105-107, 115), but there is no report on the tissue culture of Phyllostachys chinensis. Literature reports on seedling induction from external callus