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Method for culturing scindapsus aureus through terminal bud

A technology for terminal buds and green dill, which is applied in the field of using terminal buds to cultivate green dill, can solve the problems of large amount of cuttings, affecting reproduction rate, and wide area, and achieves the effect of shortening the production cycle.

Active Publication Date: 2017-04-26
东方上彩现代农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, the pothos is mainly propagated by cuttings, which require a large amount of cuttings and covers a large area. At the same time, the growing environment of the pothos is high temperature and high humidity, and the offspring of the cuttings are very susceptible to pathogens. Large-scale stem rot and root rot often occur during production. phenomenon that affects its reproductive rate

Method used

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  • Method for culturing scindapsus aureus through terminal bud

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Effect test

Embodiment 1

[0041] Step 1, selection of explants. Specifically, select a strong mother plant with normal shape and vigorous growth for pretreatment (use 80% carbendazim wettable powder 1000 times liquid to irrigate the roots, once every 10 days), cut off the 5 cm stem section terminal bud at the stem growth point, and remove it from the surface. Miscellaneous and cleaned explants were transferred to the ultra-clean workbench for disinfection treatment. This disinfection was first soaked in alcohol with a volume fraction of 75% for 15 seconds, and then soaked in 0.1wt% HgCl solution for 13 minutes. After washing with bacterial water for 3 to 5 times, sterile pothos stems are obtained.

[0042] Step 2, induction culture of callus. Specifically, the stem section of the pothos is trimmed to a length of 2cm on the ultra-clean workbench and under sterile conditions, and inoculated into the callus induction medium (including 412.5mg / L ammonium nitrate, 475mg / L potassium nitrate, 110mg / L calciu...

Embodiment 2

[0052] Step 1, selection of explants. Specifically, select a strong mother plant with normal shape and vigorous growth for pretreatment (use 80% carbendazim wettable powder 1000 times liquid to irrigate the roots, once every 10 days), cut off the 8cm stem section terminal bud at the stem growth point, and remove it from the surface. Miscellaneous and cleaned explants were transferred to the ultra-clean workbench for disinfection treatment. This disinfection was first soaked in alcohol with a volume fraction of 75% for 15 seconds, and then soaked in 0.1wt% HgCl solution for 13 minutes. After washing with bacterial water for 3 to 5 times, sterile pothos stems are obtained.

[0053] Step 2, induction culture of callus. Specifically, the stem section of the pothos is trimmed to a length of 3 cm on the ultra-clean workbench and under sterile conditions, and inoculated into the callus induction medium (including 412.5 mg / L ammonium nitrate, 475 mg / L potassium nitrate, 110mg / L calc...

Embodiment 3

[0063] Step 1, selection of explants. Specifically, a strong mother plant with normal shape and vigorous growth is selected for pretreatment (use 80% carbendazim wettable powder 1000 times liquid to irrigate the roots, once every 10 days), and the 6.5cm stem section terminal bud at the stem growth point is cut out. After removing impurities and cleaning, the explants are transferred to the ultra-clean workbench for disinfection treatment. The disinfection is first soaked in alcohol with a volume fraction of 75% for 15 seconds, and then soaked in 0.1wt% HgCl solution for 13 minutes. Use after disinfection After washing with sterile water for 3 to 5 times, sterile pothos stems are obtained.

[0064] Step 2, induction culture of callus. Specifically, the stem section of the pothos is trimmed to a length of 2.5cm on the ultra-clean workbench and under sterile conditions, and inoculated into the callus induction medium (412.5mg / L ammonium nitrate, 475mg / L potassium nitrate, 110mg...

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Abstract

The invention discloses a method for culturing scindapsus aureus through a terminal bud. The method includes the following steps that 1, the terminal bud is provided; 2, the terminal bud is subjected to inducing culture to from a callus; 3, the callus is subjected to subculture; 4, the callus after subculture is subjected to inducing culture to form clustered shoots; 5, the clustered shoots are subjected to rooting culture to form a root seedling of scindapsus aureus; 6, the root seedling of scindapsus aureus is cultivated. According to the method, with the terminal bud of scindapsus aureus being an explant material, scindapsus aureus rapidly propagates by the utilization of callus culture, there is no limit from seasons and weather, a offspring of scindapsus aureus can be effectively propagated in quantity, the production period is greatly shortened, the inheritable character of the offspring is highly identical with that of parents, and the effective way is provided for large-scale production of scindapsus aureus.

Description

technical field [0001] The invention relates to the technical field of pothos cultivation, in particular to a method for cultivating pothos by using terminal buds. Background technique [0002] The origin of pothos is in the tropical rainforest of the Solomon Islands in Indonesia. It likes a warm and humid environment, and requires loose, fertile and well-drained soil. In tropical areas, it often climbs and grows on rocks and tree trunks, becoming a huge ornamental vine, which is planted in various parts of southern my country. The pothos has strong resistance to shade, is evergreen all year round, has a thicker cuticle on the leaves, and can adapt to indoor dry environmental conditions. It is an excellent indoor foliage plant. In addition, it can also absorb and remove pollutants such as formaldehyde, benzene, and trichlorethylene in indoor air. It is a natural "air purifier" and has a great market prospect. [0003] In the prior art, the pothos is mainly propagated by cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王健刘坤申亮郑德助卢迪浩包宇飞李健施金玉
Owner 东方上彩现代农业有限公司
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