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Preparation method for preparing culture kit of umbilical cord blood mesenchemal stem cells

A technology of cell culture and mesenchymal stem cells, which is applied in the field of medicine and biology, can solve problems such as patent publications that have not yet been found, and achieve the effects of enhancing the safety of use, increasing the passage time, and reducing immunogenicity

Inactive Publication Date: 2017-04-19
SHANDONG JINGYUAN BIOTECH CO LTD
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Problems solved by technology

[0006] Through the search, no patent publications related to the patent application of the present invention have been found

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  • Preparation method for preparing culture kit of umbilical cord blood mesenchemal stem cells
  • Preparation method for preparing culture kit of umbilical cord blood mesenchemal stem cells
  • Preparation method for preparing culture kit of umbilical cord blood mesenchemal stem cells

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Embodiment Construction

[0031] A method for preparing a umbilical cord blood mesenchymal stem cell culture kit, characterized in that: the kit is composed of the following components packaged independently, 1 bottle of cell culture solution A (95 ml), 1 bottle of cell culture solution B (5 ml) and 1 vial of cell wash solution (100 ml),

[0032] Preparation of cell culture solution A: First, open the powdered DMEM medium and weigh 3.7 g of NaHCO 3 , together into 800 ml triple-distilled water, stirred until dissolved, then added 100 U / ml penicillin and 100 mg / ml streptomycin, then adjusted the pH to 7.2-7.4, then sterilized with a 0.22 μm filter, and used sterile Make up to 1000 ml with water, mix well at last, store at 4 ℃ for later use;

[0033] Preparation of cell culture medium B: Collect blood platelets collected freshly from a certain hospital and tested to meet clinical blood standards for multiple people, so that the concentration is 1.0×10 12 Cells / L, put the obtained sample in a -80°C refr...

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Abstract

The invention belongs to the technical field of medical biotechnology and relates to a preparation method for preparing a culture kit of umbilical cord blood mesenchemal stem cells. The method is characterized in that the kit is composed of the following components in separate packages: one bottle of cell culture fluid A (95 ml), one bottle of cell culture fluid B (5 ml), and one bottle of cell washing solution (100 ml). According to the technical scheme of the invention, the primary cell generation time of umbilical cord blood mesenchemal stem cells is improved. Meanwhile, even after multiple passages of umbilical cord blood mesenchemal stem cells, the totipotency of cells is still maintained. Moreover, the immunogenicity is reduced and enhance the usage safety is improved.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a method for cultivating novel umbilical cord blood mesenchymal stem cells. Background technique [0002] Cord blood mesenchymal stem cells are a kind of stem cells with self-renewal, proliferation and multi-directional differentiation potentials isolated and purified from the umbilical cord. Rejection, can avoid ethical disputes and many other advantages, and is widely used in cell therapy as an ideal cell. It has broad clinical application prospects and will become an effective means of treating human tissue and organ damage caused by aging and disease. [0003] At present, most laboratories at home and abroad still use the classic DMEM medium plus 10% fetal bovine serum to cultivate umbilical cord blood mesenchymal stem cells, and the fetal bovine serum used has the following potential risks: First, the cultured Umbilical cord mesenchymal stem cells are at risk of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/84
Inventor 崔超罗光会杜丽英王伟周国梅
Owner SHANDONG JINGYUAN BIOTECH CO LTD
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