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Lysinibacillus sp. and application thereof in pesticide degrading

A technology of Bacillus lysine, pesticides, applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of only 90.23%, the degradation rate is only 77.8%, the degradation speed is slow, etc., and achieve rapid degradation. , the effect of protecting the ecological environment and good application prospects

Active Publication Date: 2017-04-19
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the degradation rate of existing bacteria to pyrethroid pesticides and carbamate pesticides is slow. For example, the patent application with publication number CN101139559A discloses a kind of Acinetobacter, which is inoculated with 10% inoculum quality such as 20mg / L In the cypermethrin solution, after 24 hours of treatment, the degradation rate was only 77.8%. A kind of bacterium is disclosed, and its degradation rate to fenpropathrin of 100mg / L is also only 90.23% after acting for 5 days; Publication number is that when the bacteria disclosed in CN1723276A acts on carbamate compounds for 10 days, the degradation rate is only 60%. %

Method used

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  • Lysinibacillus sp. and application thereof in pesticide degrading
  • Lysinibacillus sp. and application thereof in pesticide degrading
  • Lysinibacillus sp. and application thereof in pesticide degrading

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Isolation and identification of lysine bacillus of the present invention

[0033] 1. Separation

[0034] A pesticide-degrading bacterium was screened by taking a sample of vinegar and using temperature, pH value, pesticide concentration and culture medium as enrichment conditions, and acclimatized to a variety of pesticide gradients, and the serial number was JX-5.

[0035] 2. Identification

[0036] (1) Morphological, physiological and biochemical characteristics

[0037] Morphology: Pick the bacterial strain JX-5 and culture it on LB solid medium plate, record the morphological characteristics of the colony, pick a single colony for smear, Gram staining, observe and record the color, size and shape under a 10×100 times oil microscope , Arrangement, etc.

[0038] For physiological and biochemical identification, see the eighth edition of "Berger's Handbook of Bacterial Identification".

[0039] Morphological identification of strain JX-5 (eg figure 1 ...

Embodiment 2

[0047] Example 2 Using Bacillus lysinus of the present invention to degrade pesticides in soil

[0048] Take Lysinibacillus sp. JX-5 of the present invention, the preservation number is CGMCC No. 13142, referred to as strain JX-5. Strain activation: pick the strain JX-5 into the LB liquid medium, 30°C, 180r / min shaking culture for 24h; then continuously streak, pick a single colony for two cultures (30°C), pick a single colony to inoculate into The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 6 cfu / mL, made into seed solution.

[0049] Add the seed liquid to the soil to be treated, mix well, and that's it.

Embodiment 3

[0050] Example 3 Using Bacillus lysinus of the present invention to degrade pesticides in water

[0051] Take Lysinibacillus sp. JX-5 of the present invention, the preservation number is CGMCC No. 13142, referred to as strain JX-5. Strain activation: pick the strain JX-5 into the LB liquid medium, 30°C, 180r / min shaking culture for 24h; then continuously streak, pick a single colony for two cultures (30°C), pick a single colony to inoculate into The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 8 cfu / mL, made into seed solution.

[0052] Add the seed liquid into the water to be treated, mix well, and you are done.

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Abstract

The invention discloses lysinibacillus sp., which is lysinibacillus sp.JX-5 (preservation number: CGMCC No.13142) preserved in the China General Microbiological Culture Collection Center. The lysinibacillus sp.JX-5 (preservation number: CGMCC No.13142) of the invention can effectively degrade pyrethriod pesticides and carbamate pesticides, can be used in bioremediation or biological purification of soil and water polluted by pyrethriod pesticides and carbamate pesticides, can protect the ecological environment, and has an excellent application prospect.

Description

technical field [0001] The invention relates to a strain of lysine bacillus and its application in degrading pesticides. Background technique [0002] Cypermethrin (CY) is one of the most commonly used varieties of pyrethroid pesticides, and it is widely used in the prevention and control of pests in tea trees, fruits and vegetables, humans and animals. Due to its strong hydrophobicity and light and heat stability, its natural degradation rate is slow, and its half-life in the environment is as long as 94.2d-1103d, which easily leads to its residual accumulation in the environment. Therefore, it is widely used in soil, water, agricultural and livestock products. Higher detection rate and exceeding rate often appear in the test. Cypermethrin is highly toxic to fish and other aquatic animals. It can also accumulate in animals and plants, has acute toxicity to the nervous system, and can damage the development of the male reproductive system. Long-term exposure may also cause ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/04C12R1/07
CPCA62D3/02A62D2101/04C12N1/205C12R2001/07
Inventor 李建龙代敏龙娜娜蒋蔓
Owner CHENGDU MEDICAL COLLEGE
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