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A kind of extraction method of different forms of polyphenols in camellia oleifera seed

A camellia seed and extraction technology, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, antidote and other directions to achieve the effects of simplified operation steps, less dosage and high extraction efficiency

Active Publication Date: 2020-04-21
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art does not provide an efficient extraction method for simultaneously extracting different forms of polyphenols in Camellia oleifera seeds

Method used

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  • A kind of extraction method of different forms of polyphenols in camellia oleifera seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Degrease the picked ripe camellia seed by Soxhlet extraction after drying, shelling and crushing to obtain defatted camellia seed powder with an oil content of <2%;

[0043] (2) Mix 6mL extractant (methanol / acetone / water solution with a volume ratio of 8 / 6 / 6) and 4g defatted camellia oleifera seed powder, add it into a sealed extraction kettle, control the temperature in the reaction kettle to be 70°C, and the pressure to be 95Mpa, After extracting for 15 minutes, the pressure was released, and the above operation was repeated for a total of 5 times to obtain the extraction solution and the precipitation of camellia oleifera seeds;

[0044] (3) The Camellia oleifera seed precipitation that above-mentioned step 2) is obtained adds the NaOH of 4mol / L under nitrogen blowing condition and carries out alkali hydrolysis 5h, carries out the pH value of the product after adjusting described alkali hydrolysis with the hydrochloric acid of 6mol / L to 1.5, Centrifuge at 11000 r...

Embodiment 2

[0047] (1) Soxhlet extraction is used to degrease the mature camellia oleifera seeds after picking in the sun, shelling and crushing to obtain defatted camellia oleifera seed powder with an oil content of <1.5%;

[0048] (2) Mix 10mL extractant (methanol / acetone / water solution with a volume ratio of 8 / 8 / 6) and 4g defatted camellia oleifera seed powder, add it to a sealed extraction kettle, control the temperature in the reaction kettle to be 85°C, and the pressure to be 98Mpa, After extracting for 15 minutes, the pressure was released, and the above operation was repeated 4 times in total to obtain the extraction solution and the precipitation of camellia oleifera seeds;

[0049] (3) The Camellia oleifera seed precipitation that above-mentioned step 2) is obtained adds the NaOH of 4mol / L under nitrogen blowing condition and carries out alkali hydrolysis 3h, carries out the pH value of the product after adjusting described alkali hydrolysis with the hydrochloric acid of 6mol / L t...

Embodiment 3

[0052] (1) Soxhlet extraction is used to degrease the mature camellia oleifera seeds after picking in the sun, shelling and crushing to obtain defatted camellia oleifera seed powder with an oil content of less than 1%;

[0053] (2) Mix 6mL of extractant (methanol / acetone / water solution with a volume ratio of 8 / 6 / 6) and 3g of defatted camellia oleifera seed powder, add it to a sealed extraction kettle, control the temperature in the reaction kettle to 90°C, and the pressure to 100Mpa, After extracting for 10 minutes, release the pressure, repeat the above operation for a total of 6 times, and obtain the extraction solution and the precipitation of camellia oleifera seeds;

[0054] (3) The Camellia oleifera seed precipitation that above-mentioned step 2) is obtained adds the NaOH of 4mol / L under nitrogen blowing condition and carries out alkali hydrolysis 4h, carries out the pH value of the product after adjusting described alkali hydrolysis with 6mol / L hydrochloric acid to be 2,...

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Abstract

The present invention relates to an extraction method for extracting different forms of polyphenol in camellia seeds and belongs to the technical field of natural product extraction. The extraction method provided in the invention comprising the steps of 1) extracting camellia seeds after being defatted and crushed so as to obtain an extract solution and a camellia seed precipitate; 2) subjecting the camellia seed precipitate to alkali hydrolysis, carrying out solid-liquid separation, extracting a supernatant obtained after the solid-liquid separation, and concentrating an obtained extract to obtain camellia seed insoluble phenolic acids; 3) obtaining an extraction solution, extracting the extraction solution with ethyl acetate to obtain an upper-layer extract phase and a lower-layer raffinate phase, concentrating the upper-layer extract phase to obtain camellia seed free phenolic acids, subjecting the lower-layer raffinate phase to alkali hydrolysis and extraction, and concentrating to obtain camellia seed combined-state phenolic acids. Based on the extraction method, different forms of polyphenol can be obtained simultaneously. Meanwhile, the application amount of organic solvents is small and the concentration of polyphenol is high.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to an extraction method of polyphenols in different forms in camellia oleifera seeds. Background technique [0002] Camellia oleifera is a unique native tree species with excellent economic and ecological benefits in my country. Camellia oleifera seeds contain more than 60% oil, and are rich in vitamins, trace elements, sterols, squalene, carotene, polyphenols and other nutrients. Polyphenols are known as the "seventh type of nutrient". They have the functions of scavenging free radicals, inhibiting lipid peroxidation, and enhancing the effects of other antioxidants. Scholars pay attention. Efficient extraction and analysis of polyphenols in camellia oleifera is of great significance for a comprehensive understanding of polyphenol components in camellia oleifera seeds. There are three forms of polyphenols in plants: free state, esterified state, and bound state...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/82A61P39/06A61K131/00
CPCA61K36/82A61K2236/333A61K2236/35A61K2236/37A61K2236/39A61K2236/53
Inventor 罗凡费学谦许伟戚雨婷郭少海
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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