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Biomimetic mineralized adhesive film prepared from polymer film forming material-loaded ACP and in-vitro induction remineralization method

A film-forming material and biomimetic mineralization technology, applied in dental prostheses, pharmaceutical formulas, dental preparations, etc., can solve problems such as uncertain actual effects, and achieve the effects of wide application, easy use, and low cost

Active Publication Date: 2017-03-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ACP cannot deliver ACP to enamel or dentin in a long-term and directional manner in solutions or pastes (such as mouthwash or tooth mousse), providing a long-term source of calcium and phosphorus, resulting in uncertain actual effects

Method used

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  • Biomimetic mineralized adhesive film prepared from polymer film forming material-loaded ACP and in-vitro induction remineralization method
  • Biomimetic mineralized adhesive film prepared from polymer film forming material-loaded ACP and in-vitro induction remineralization method
  • Biomimetic mineralized adhesive film prepared from polymer film forming material-loaded ACP and in-vitro induction remineralization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0047] Embodiment 1: The preparation of HPMC-loaded ACP biomimetic mineralization film is as follows: add HPMC powder to 70% ethanol aqueous solution at a ratio of 1:6, and after it is completely swollen into a glue, then add 800 μg / ml PAA-stabilized ACP at a ratio of 1:4 Add to HPMC sol and stir well. After the air bubbles in the sol are removed by ultrasonic or vacuum, apply a thin layer evenly on the flat plate, put it in an oven to dry, cool, and form a film, and cut it into films of different sizes.

[0048] Before applying the HPMC-loaded ACP film, the phase transition of ACP in the mineralized film was characterized by Fourier transform infrared spectroscopy (FTIR). The result is as figure 1 As shown, in the infrared spectrum of ACP, at 534cm -1 and 1100cm -1 The absorption peak at PO4 3- cause, of which 534cm -1 The absorption peak at the position does not split, indicating that the measured sample is not crystallized and is an amorphous phase, indicating that ACP...

Embodiment approach 2

[0051] Embodiment 2: Preparation of chitosan-loaded ACP biomimetic mineralization film: add chitosan powder to pure aqueous solution at a ratio of 1:5, and after it is completely swollen into a glue, add 4mg / ml PAsp stabilized ACP at a ratio of 1:5 1 Add to the sol and mix well. After the air bubbles in the sol are removed by ultrasonic or vacuum, apply a thin layer evenly on the flat plate, put it in an oven to dry, cool, and form a film, and cut it into films of different sizes. Fourier transform infrared spectroscopy (FTIR) characterization is the same as scheme 1.

[0052] Preparation of demineralized tooth enamel: Select a freshly extracted human third molar without caries, defects, and cracks for the experiment, store it in 0.5% chloramine solution, and use it within one month. Under running water, 70 pieces of enamel slices with a size of about 5mm*5mm*1mm were prepared on the buccal and lingual sides parallel to the long axis of the crown with an Isomet low-speed micr...

Embodiment approach 3

[0054] Embodiment 3: Preparation of KGM-loaded ACP biomimetic mineralization film: add KGM powder to absolute ethanol at a ratio of 1:9, and after it is completely swollen into a glue, add polyelectrolyte-stabilized ACP and L-glutamic acid to simulate NCPs , the mass ratio of L-glutamic acid to polyelectrolyte-stabilized ACP is 1:3 and fully stirred and mixed. After the air bubbles in the sol are removed by ultrasonic or vacuum, apply a thin layer evenly on the flat plate, put it in an oven to dry, cool, and form a film, and cut it into films of different sizes.

[0055] Preparation of demineralized dentin: Select a freshly extracted human third molar without caries, defects, and cracks for the experiment, store it in 0.5% chloramine solution, and use it within one month. Under running water, 70 dentin slices with a size of about 3*2*1mm were prepared in the middle of the crown perpendicular to the long axis of the crown with an Isomet low-speed microtome. 37% phosphoric acid...

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Abstract

The invention discloses a biomimetic mineralized adhesive film prepared from polymer film forming material-loaded ACP and an in-vitro induction remineralization method. One or more of polymer film forming materials such as hydroxypropylmethylcellulose, chitosan and konjac glucomannan are added into an ethanol aqueous solution or pure water or anhydrous ethanol, one or more stable ACPs of polyelectrolytes such as polyacrylic acid and polyaspartic acid are added into the sol after complete swelling for sol slurry formation, one or more of non-collagen analogues such as glutamic acid and citric acid are added into the mixed sol, the mixture is uniformly stirred so that all the bubbles are removed, a glass slide is uniformly coated with a thin layer of the mixture, and the glass slide with the mixture is put into an oven and is dried and cooled so that the film is formed. The polymer film-forming material carries ACP so that the biomimetic mineralized adhesive film is obtained. The biomimetic mineralized adhesive film can be dried and stored for a long time, can induce re-mineralization of recombinant type I collagen, demineralized enamel and dentin, is easy to use, has a low cost and can be attached to any tooth position needing to be mineralized. The method provides a novel technical method for remineralization repair.

Description

technical field [0001] The invention relates to the technical field of biomimetic biomaterial engineering, in particular to the field of biomineralization, in particular to a polymer film-forming material loaded with amorphous calcium phosphate (amorphous calcium phosphate, ACP) biomimetic mineralization film and a method for inducing remineralization in vitro. Background technique [0002] The formation and mineralization of enamel and dentin are typical biomineralization, that is, nanocrystalline particles are precursors induced by odontoblasts, through agglomeration, self-assembly, and mesogenic transformation from unstable precursors. The process of generating hydroxyapatite (Hydroxyapatite, HAp) crystals. [0003] At present, regarding the remineralization of tooth enamel [1] scholars at home and abroad use amelogenin, peptides and various organic polymer surfactants to synthesize hydroxyapatite with a tooth-like structure, and apply these technologies to damaged tooth ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/033A61K6/097A61K6/838A61K6/898
CPCA61K6/838A61K6/898C08L1/284
Inventor 傅柏平王喆陈怡范佳燕吴志芳徐婧秋李明星金晓婷
Owner ZHEJIANG UNIV
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