Breeding method of new transgenic herbicide-resistant and high-temperature-resistant cotton strain
A herbicide resistance and cultivation method technology, applied in the direction of plant genetic improvement, botanical equipment and methods, application, etc., can solve the problems of reduced pollen quantity and vigor, difficulty in artificial hybrid pollination, affecting the production of hybrid cotton seeds, etc. high temperature resistance
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Embodiment 1
[0023] A method for cultivating a new strain of transgenic herbicide-resistant and high-temperature-resistant cotton, the specific steps of which are:
[0024] A. In 2007, 10 genetically modified herbicide-resistant cotton lines including Y16, Y17, Y18, Y19, Y20, Y21, Y22, Y23, Y24, and Y25 were introduced from Genesis Company, and each p line was planted in 1 ridge. About 25 plants are planted for each material. When the cotton plant has 5 leaves and 1 heart, the cotton growth point is sprayed with a glyphosate solution with a concentration of 700ug / mL, and the herbicide-resistant cotton plants are marked with wool. The glyphosate solution with a concentration of 700ug / mL was screened for 5 generations in continuous breeding in Hunan and Hunan; after 5 generations, stable herbicide-resistant varieties H01, H02, H03, H04, H05, H06, H07, H08, H09, H10;
[0025] Table 1 Herbicide resistance of H01, H02, H03, H04, H05, H06, H07, H08, H09, H10
[0026]
[0027]
[0028] B....
Embodiment 2
[0037] In 2014, the herbicide-resistant and high-temperature-resistant cotton line CH4 was used as the female parent, and the boll-setting transgenic insect-resistant cotton F3 was used as the male parent. ZHM19 was obtained in the 2015 Hunan Cotton Science Research 2015 Excellent Line Comparative Test for seed cotton yield. Winning the first place, ZHM19 was recommended to participate in the 2016 National Cotton Regional Test.
[0038] ZHM19 and traits of each parent
[0039]
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