Ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for nutrition enhancer in milk and dairy product

Abstract

Belonging to the technical field of food safety detection, the invention discloses an ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry screening method for a nutrition enhancer in milk and dairy product. The method includes: utilizing optimized QuEChERS method to perform pretreatment on a sample; using ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry for screening, adopting full scanning / data dependent acquisition, full scanning / variable data non-dependent acquisition, and full scanning / all-ion fragmentation acquisition scanning modes, selecting positive ion or negative ion mode scanning to obtain complete first-order and second-order spectrum; applying Exact Finder 2.5 software to process the obtained first-order and second-order spectrum, and extracting compound information; and comparing the information in the established spectrum library with the screening result of the detected object, and confirming the composition of the nutrition enhancer in the milk and dairy product. The mass spectrometer used in the invention has the main advantages of high resolution, accurate qualitative and quantitative result, high sensitivity and high quality precision, etc., and can be applied to analysis of complex matrices.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of food safety detection, and in particular relates to an ultra-high performance liquid chromatography-quadrupole electrostatic field orbital ion trap mass spectrometry screening method for nutrition enhancers in milk and dairy products. [0003] 【Background technique】 [0004] Nutritional fortifiers refer to natural or synthetic substances belonging to the scope of natural nutrients that are added to foods to enhance nutritional components. Foods that have been fortified are called fortified foods. The nutritional fortifiers allowed to be used in my country include the types stipulated in GB14880 and GB2760. Nutritional enhancers are mainly divided into vitamins, unsaturated fatty acids, nucleosides and nucleotides. Adding a certain amount of nutritional fortifiers to milk and dairy products can balance the nutrients and make up for the lack of nutrients. In recent years, people have paid more and...

AI Technical Summary

Problems solved by technology

However, at present, there are few high-throughput and rapid detection methods for exogenous risk substances in milk and dairy products. The pre-treatment operations are complicated, and the cost of materials and equipment is high. They are limited to the rapid detection of several or one type of compounds.

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