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Immobilized microbial repairing agent for spilled oil and preparation method thereof

A technology for immobilizing microorganisms and repairing agents, applied to chemical instruments and methods, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., can solve the problem of strong agglomeration of polyvinyl alcohol and reduced Microbial activity, low mechanical strength of preparations, etc., to achieve good stability and reusability, improve mechanical strength, high microbial activity and cell capacity

Inactive Publication Date: 2017-03-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And compared to sodium alginate-CaCl 2 Embedding method, polyvinyl alcohol-H 3 BO 3 Although the prepared immobilized preparation has high mechanical strength and long service life, it is difficult for polyvinyl alcohol to form into balls due to strong agglomeration, and the saturated boric acid solution used for cross-linking polyvinyl alcohol is highly toxic to microorganisms and will Significantly reduces the activity of microorganisms after immobilization
Sodium Alginate-CaCl 2 Although the embedding method has mild reaction conditions, the embedding material is non-toxic to organisms, and the embedded cells have high activity, the mechanical strength of the preparation is low and the mass transfer is poor.
Therefore, both have their own advantages and disadvantages, and cannot achieve a relatively ideal state.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]Preparation of hemp stalk-based activated carbon: clean, sieve, dry and pulverize raw hemp stalks, pass through a 25-mesh sieve, and set aside. Weigh the crushed hemp stalks above, add them into the citric acid solution with a mass fraction of 5% according to the mass ratio of the hemp stalks to the citric acid solution of 1:2, stir and mix well, and soak at room temperature for 1.5h. Then put the impregnated material liquid into the muffle furnace, heat up to 350°C at a heating rate of 40°C / min for 90 minutes for activation. Take the activated sample out of the muffle furnace, and after cooling, pour the sample into a sodium hydroxide solution prepared by 10mol / L sodium hydroxide and water at a volume ratio of 1:8 and soak for 15 minutes. Then wash the sample with hot water at 70°C until the pH is neutral, put the washed sample in an electric blast oven, dry at 100°C for 3 hours, take it out and put it in a desiccator to cool. Finally, the obtained activated sample was...

Embodiment 2

[0039] Preparation of hemp stalk-based activated carbon: clean, sieve, dry and pulverize raw hemp stalks, pass through a 30-mesh sieve, and set aside. Weigh the above crushed hemp stalks, add the hemp stalks to the citric acid solution at a mass ratio of 1:3 into the citric acid solution with a mass fraction of 8%, stir and mix well, and soak at room temperature for 1 hour. Then put the impregnated material liquid into the muffle furnace, and raise the temperature to 400°C at a heating rate of 45°C / min for 80 minutes of activation. Take the activated sample out of the muffle furnace, and after cooling, pour the sample into a sodium hydroxide solution prepared by 10mol / L sodium hydroxide and water at a volume ratio of 1:8 and soak for 20min. Then wash the sample with hot water at 75°C until the pH is neutral, put the washed sample in an electric blast oven, dry it at 105°C for 4 hours, take it out and put it in a desiccator to cool. Finally, the obtained activated sample was p...

Embodiment 3

[0043] Preparation of hemp stalk-based activated carbon: clean, sieve, dry and pulverize raw hemp stalks, pass through a 30-mesh sieve, and set aside. Weigh the above crushed hemp stalks, add them into the citric acid solution with a mass fraction of 10% according to the mass ratio of the hemp stalks to the citric acid solution of 1:3, stir and mix well, and soak at room temperature for 1 hour. Then put the impregnated material liquid into the muffle furnace, raise the temperature to 500°C at a heating rate of 50°C / min and keep it activated for 60 minutes. Take the activated sample out of the muffle furnace, and after cooling, pour the sample into a sodium hydroxide solution prepared by 10mol / L sodium hydroxide and water at a volume ratio of 1:9 and soak for 20min. Then wash the sample with hot water at 75°C until the pH is neutral, put the washed sample in an electric blast oven, dry it at 110°C for 4 hours, take it out and put it in a desiccator to cool. Finally, the obtain...

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PUM

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Abstract

The invention relates to an immobilized microbial repairing agent for spilled oil and a preparation method thereof. The repairing agent is prepared by embedding microbes with a sodium alginate-CaCl2-biobased active carbon, wherein the concentration of the embedded microbes is 8*10<8> to 6*10<9> cell / g. The preparation method comprises the following steps: mixing biobased active carbon with microbial seed liquid for adsorption so as to from a mixed suspension; adding a sterilized sodium alginate solution with a temperature of 30 to 40 DEG C into the mixed suspension and carrying out uniform mixig so as to obtain a viscous mixed solution; and subjecting the viscous mixed solution to dispersion and balling in a CaCl2 solution and carrying out cross-linking for 12 to 24 h so as to obtain the immobilized microbial repairing agent for spilled oil. The preparation method is simple and easy to operate and mild in reaction conditions; and the prepared immobilized microbial repairing agent is not prone to microbial leakage, has good stability and reusability and high microbe activity and cell volume and has a petroleum hydrocarbon degradation rate of 81 to 95%.

Description

technical field [0001] The invention relates to a microbial restoration agent and a preparation method thereof, in particular to an immobilized microbial oil spill restoration agent and a preparation method thereof. Background technique [0002] With the rapid development of offshore oil development and marine transportation, oil spill accidents caused by blowouts, oil spills from transport ships, shipwrecks, and oil pipeline leaks have become increasingly frequent. After the oil spill accident, although some surface oil can be removed by physical methods (such as fence recovery, etc.) and chemical methods (such as spraying dispersants, etc.), the remaining petroleum hydrocarbons remain in the marine environment for a long time, which is harmful to the ocean. Ecosystems and human health can cause enormous and lasting damage. [0003] With the deepening of research, researchers at home and abroad have found that there are more than 200 kinds of hydrocarbon-degrading microorg...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34C02F101/32
CPCC02F3/344C02F2101/32C12N11/04C12N11/10C12N11/14
Inventor 帅京妤陈庆国刘梅王亚超
Owner ZHEJIANG OCEAN UNIV
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