Preparation method of small fish protein peptide chelated zinc
A small peptide chelation and fish protein technology, which is applied in the field of fish protein small peptide chelated zinc preparation, can solve the problems of long chelation time, low chelation rate, and difficult control of the chelation process, and achieve short chelation time , High chelation rate, effective control of the effect of the chelation process
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Embodiment 1
[0026] (1) After dissolving small fish protein peptides, adjust the pH to 5.0-5.5 to obtain a small peptide solution. The mass percentage of the small peptide solution is 20%.
[0027] (2) Add zinc chloride to the small peptide solution, the mass ratio of zinc chloride to small peptide solution is 1:2, fully dissolve, heat up to 35°C, ultrasonically react for 15min, ultrasonic time is 5 seconds, and the interval The time is 2 seconds, and the ultrasonic power is 70%.
[0028] (3) Concentrate the reaction solution to 40% of the original volume after filtration, and obtain fish protein small peptide chelated zinc after spray drying.
[0029] After testing, the chelation rate of fish protein small peptide chelated zinc in this example was 98.2%, and the zinc content was 15.7%.
[0030] According to the preparation method in Yang Huicheng's "A Preparation Process for Fish Skin Collagen Polypeptide Chelated Zinc", the chelation rate of fish skin collagen polypeptide and zinc is 85...
Embodiment 2
[0031] Example 2 Study on the Effect of Small Peptide Chelated Zinc Supplement
[0032] As a zinc deficiency model in mice, the dosage of zinc sulfate group, zinc gluconate, and small peptide chelated zinc was 7.00 mgZn / kg.bw per day, and it was administered orally for 14 consecutive days. Compared with the zinc deficiency group, the small peptide zinc group had a significant difference. Under the same zinc supplementation dose, the serum zinc content of the mice was small peptide zinc group > zinc gluconate group > zinc sulfate group.
[0033] Table 1 Study on the effect of small peptide chelated zinc supplementation
[0034] group Zinc deficiency group small peptide zinc group Zinc gluconate group Zinc sulfate group Zinc content (µg / g) 28.92±1.37 37.09±0.52** 34.30±1.22 32.51±1.53
[0035] Note: Compared with zinc deficiency group, *p<0.05, **p<0.01.
Embodiment 3
[0036] Example 3 Research on Antioxidant Effect of Small Peptide Chelated Zinc
[0037] As a zinc deficiency model in mice, the dosage of zinc sulfate group, zinc gluconate, and small peptide chelated zinc was 7.00 mgZn / kg.bw per day, and it was administered orally for 14 consecutive days. Determination of total antioxidant capacity in mouse liver tissue. Compared with the zinc deficiency group, the small peptide zinc group had a significant difference. Under the same zinc supplementation dose, the order of the total antioxidant capacity of the mouse liver was small peptide zinc group > zinc gluconate group > zinc sulfate group. It shows that under the condition of the same zinc dosage, small peptide zinc is better than zinc gluconate and zinc sulfate.
[0038] Table 2 Research on the antioxidant effect of small peptide chelated zinc
[0039] total antioxidant capacity Zinc deficiency group small peptide zinc group Zinc gluconate group Zinc sulfate group ...
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